PC‑1 NF suppresses high glucose‑stimulated inflammation and extracellular matrix accumulation in glomerular mesangial cells via the Wnt/β‑catenin signaling

Xiao,Liangxiang; Chen,Yingying; Yuan,Yang; Xu,Bo; Gao,Qing; Chen,Ping; Zhang,Tianying; Guan,Tianjun

doi:10.3892/etm.2019.7793

PC‑1 NF suppresses high glucose‑stimulated inflammation and extracellular matrix accumulation in glomerular mesangial cells via the Wnt/β‑catenin signaling

Authors:
- Liangxiang Xiao
- Yingying Chen
- Yang Yuan
- Bo Xu
- Qing Gao
- Ping Chen
- Tianying Zhang
- Tianjun Guan
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Affiliations: Department of Nephrology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian 361004, P.R. China, Department of Nephrology, Ningbo First Hospital, Ningbo, Zhejiang 315010, P.R. China
Published online on: July 19, 2019 https://doi.org/10.3892/etm.2019.7793
Pages: 2029-2036
Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy (DN) is the leading cause of end‑stage renal disease worldwide with high morbidity and mortality. Glomerular mesangial cell (MC) proliferation, inflammatory cell infiltration and extracellular matrix (ECM) accumulation are the main pathological characteristics of DN. A previous study revealed that polycystin‑1 N‑terminal fragment (PC‑1 NF) fusion protein could inhibit ECM accumulation in a mesangial proliferative glomerulonephritis model. However, the role of PC‑1 NF fusion protein in DN remains unknown. The results of the present study indicated that PC‑1 NF fusion protein significantly abolished high glucose (HG)‑induced glomerular MC viability over three time points measured (24, 48 and 72 h). In addition, PC‑1 NF suppressed the levels of monocyte chemotactic peptide‑1 and tumor necrosis factor α, as well as the expression of fibronectin and collagen IV, in HG‑stimulated MCs. Furthermore, PC‑1 NF fusion protein efficiently inhibited the activation of Wnt/β‑catenin signaling pathway in HG‑stimulated MCs. Taken together, these data indicated that PC‑1 NF fusion protein inhibited HG‑induced MC proliferation, inflammation, and ECM expression via the modulation of the Wnt signaling pathway. The present study indicated that PC‑1 NF fusion protein may be a potential agent in treating DN.

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