Expression of β‑catenin and MMP‑8 in gingival crevicular fluid and gingival tissue indicates the disease severity
of patients with chronic periodontitis

Zhu,Lilei; Yao,Yao; Liu,Jian; Wang,Jingyu; Xie,Hui

doi:10.3892/etm.2019.7794

Expression of β‑catenin and MMP‑8 in gingival crevicular fluid and gingival tissue indicates the disease severity of patients with chronic periodontitis

Authors:
- Lilei Zhu
- Yao Yao
- Jian Liu
- Jingyu Wang
- Hui Xie
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Affiliations: Department of Periodontology, Changsha Stomatological Hospital, Changsha, Hunan 410005, P.R. China, Department of Orthodontics, Changsha Stomatological Hospital, Changsha, Hunan 410005, P.R. China, Department of Endodontics, Changsha Stomatological Hospital, Changsha, Hunan 410005, P.R. China
Published online on: July 19, 2019 https://doi.org/10.3892/etm.2019.7794
Pages: 2131-2139
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the interaction among β‑catenin, matrix metalloproteinase‑8 (MMP‑8) and severity in patients with chronic periodontitis. Both gingival crevicular fluid (GCF) and gingival tissue was collected from 21 healthy control individuals, 21 patients with moderate chronic periodontitis (mCP) and 23 patients with severe chronic periodontitis (sCP). The concentration of MMP‑8 in GCF was detected via ELISA and the mRNA levels of β‑catenin and MMP‑8 in GCF and gingival tissue was detected via reverse transcription‑quantitative PCR. The protein levels of β‑catenin and MMP‑8 in gingival tissue was detected using western blotting and the interaction between β‑catenin and MMP‑8 in gingival tissue was detected by co‑immunoprecipitation. The expression of β‑catenin and MMP‑8 was significantly higher in the GCF and gingival tissue of patients with chronic periodontitis (mCP and sCP) compared with the control patients. Furthermore, the expression of β‑catenin and MMP‑8 in GCF and gingival tissue was positively correlated with the clinical attachment level. In addition, a positive interaction was identified between β‑catenin and MMP‑8, and the expression of β‑catenin was positively correlated with the expression of MMP‑8 in GCF and gingival tissue. The CGF and gingival tissue expression of β‑catenin and MMP‑8 may indicate disease severity in patients with chronic periodontitis.

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