Silencing of long non‑coding RNA NCK1‑AS1 inhibits cell proliferation and migration via inhibition of microRNA‑134 in cervical cancer

Huang,Lu; Gan,Xiaoqin; He,Li; Wang,Luying; Yu,Jie

doi:10.3892/etm.2019.7799

Silencing of long non‑coding RNA NCK1‑AS1 inhibits cell proliferation and migration via inhibition of microRNA‑134 in cervical cancer

Authors:
- Lu Huang
- Xiaoqin Gan
- Li He
- Luying Wang
- Jie Yu
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Affiliations: Department of Obstetrics and Gynaecology, Chengdu Women and Children's Central Hospital, Chengdu, Sichuan 610031, P.R. China
Published online on: July 19, 2019 https://doi.org/10.3892/etm.2019.7799
Pages: 2314-2322

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Abstract

Long non‑coding RNA NCK1‑antisense 1 (AS1) has recently been demonstrated to promote cell proliferation and induce cell cycle progression through the crosstalk NCK1‑AS1/microRNA (miR)‑6857/cyclin dependent kinase 1 pathway in cervical cancer. However, the regulatory mechanism of NCK1‑AS1 in cervical cancer growth and metastasis remains largely unclear. In the present study, it was identified that NCK1‑AS1 was significantly upregulated in cervical cancer tissues compared with the levels in adjacent non‑tumour tissues. High expression levels of NCK1‑AS1 were associated with tumour progression and poor prognosis in patients with cervical cancer. Silencing of NCK1‑AS1 expression significantly decreased the levels of proliferation and migration of cervical cancer cells, and decreased the protein expression levels of matrix metalloproteinase (MMP)‑2 and MMP‑9. The results of the luciferase reporter gene assay indicated that there was an miR‑134 binding site within the NCK1‑AS1 gene in cervical cancer cells. miR‑134 was significantly downregulated in cervical cancer tissues compared with the miR‑134 levels in adjacent non‑tumour tissues, and the expression level of miR‑134 was inversely correlated with the NCK1‑AS1 expression levels in cervical cancer tissues. Knockdown of miR‑134 attenuated the inhibitory effects of NCK1‑AS1 downregulation on the proliferation and migration of cervical cancer cells. Therefore, the data from the present study suggested that NCK1‑AS1 serves a promotive role in cervical cancer cell proliferation and migration by functioning as a molecular sponge for miR‑134. NCK1‑AS1 may become a novel therapeutic target for cervical cancer.

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