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Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model

  • Authors:
    • Huiting Zhou
    • Huihui Song
    • Yi Wu
    • Xiang Liu
    • Jing Li
    • He Zhao
    • Miaomiao Tang
    • Xiaoyuan Ji
    • Lu Zhang
    • Yuanyuan Su
    • Yao He
    • Kehong Feng
    • Yang Jiao
    • Hua Xu
  • View Affiliations / Copyright

    Affiliations: Institute of Pediatric Research, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China, Department of Medical Imaging, Suzhou Psychiatric Hospital, The Affiliated Guangji Hospital of Soochow University, Suzhou, Jiangsu 215137, P.R. China, Department of Pathology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China, Department of Ophthalmology, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China, Laboratory of Nanoscale Biochemical Analysis, Jiangsu Key Laboratory for Carbon‑Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, P.R. China, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2037-2050
    |
    Published online on: July 26, 2019
       https://doi.org/10.3892/etm.2019.7819
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Abstract

Retinopathy of prematurity (ROP) is a leading cause of childhood blindness. At present, the molecular mechanisms underlying ROP are still far from being clearly understood. Circular RNAs (circRNAs), a novel class of noncoding RNAs, have been reported to serve vital regulatory roles in several human diseases. However, it is still unclear how circRNAs are involved in ROP. In the present study, oxygen‑induced retinopathy (OIR) murine retinal samples and paired normal tissues were chosen for high‑throughput transcriptome RNA sequencing and bioinformatic analyses. As a result, a total of 236 differentially expressed circRNAs, 14 differentially expressed miRNAs, and 9,756 differentially expressed mRNAs were identified in the OIR samples. Gene ontology analysis showed that angiogenesis ranked in the top five upregulated biological processes associated with differential mRNA expression. Then, 66 co‑expression pairs of circRNA‑mRNA were predicted according to the mRNAs that were enriched in angiogenesis. Furthermore, coregulation prediction was separately performed to identify the differentially expressed miRNAs that targeted angiogenesis‑associated circRNAs or mRNAs. Finally, nine differentially expressed circRNAs were predicted to be competing endogenous RNAs by constructing a circRNA‑miRNA‑mRNA network followed by reverse transcription‑quantitative PCR validation. The results of the present study suggest that the identified set of circRNA transcripts and the potential regulatory mechanisms for the development of ROP are worthy of functional studies.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou H, Song H, Wu Y, Liu X, Li J, Zhao H, Tang M, Ji X, Zhang L, Su Y, Su Y, et al: Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model. Exp Ther Med 18: 2037-2050, 2019.
APA
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H. ... Xu, H. (2019). Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model. Experimental and Therapeutic Medicine, 18, 2037-2050. https://doi.org/10.3892/etm.2019.7819
MLA
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H., Tang, M., Ji, X., Zhang, L., Su, Y., He, Y., Feng, K., Jiao, Y., Xu, H."Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model". Experimental and Therapeutic Medicine 18.3 (2019): 2037-2050.
Chicago
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H., Tang, M., Ji, X., Zhang, L., Su, Y., He, Y., Feng, K., Jiao, Y., Xu, H."Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model". Experimental and Therapeutic Medicine 18, no. 3 (2019): 2037-2050. https://doi.org/10.3892/etm.2019.7819
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou H, Song H, Wu Y, Liu X, Li J, Zhao H, Tang M, Ji X, Zhang L, Su Y, Su Y, et al: Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model. Exp Ther Med 18: 2037-2050, 2019.
APA
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H. ... Xu, H. (2019). Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model. Experimental and Therapeutic Medicine, 18, 2037-2050. https://doi.org/10.3892/etm.2019.7819
MLA
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H., Tang, M., Ji, X., Zhang, L., Su, Y., He, Y., Feng, K., Jiao, Y., Xu, H."Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model". Experimental and Therapeutic Medicine 18.3 (2019): 2037-2050.
Chicago
Zhou, H., Song, H., Wu, Y., Liu, X., Li, J., Zhao, H., Tang, M., Ji, X., Zhang, L., Su, Y., He, Y., Feng, K., Jiao, Y., Xu, H."Oxygen‑induced circRNA profiles and coregulatory networks in a retinopathy of prematurity mouse model". Experimental and Therapeutic Medicine 18, no. 3 (2019): 2037-2050. https://doi.org/10.3892/etm.2019.7819
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