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Article

Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE

  • Authors:
    • Hongwei Liu
    • Jian Zhang
    • Pingping Zhou
    • Hao Sun
    • Martha Katsarou
    • Nikolaos Drakoulis
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P.R. China, Department of Ophthalmology, The Huai'an Affiliated Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223002, P.R. China, Research Group of Clinical Pharmacology and Pharmacogenomics, Faculty of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, 15771 Athens, Greece
  • Pages: 5072-5077
    |
    Published online on: September 16, 2019
       https://doi.org/10.3892/etm.2019.8009
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Abstract

Conjunctivitis associated systemic lupus erythematosus (caSLE) is a connective tissue autoimmune disease with a large spectrum of clinical manifestations. The disease may lead to ocular complications and in severe cases may be sight‑threatening. This study investigated the expression of vascular adhesion protein‑1 (VAP‑1) in SLE patients with conjunctivitis and the relationship between VAP‑1 and other proteins in the onset of the disease. Ten patients with caSLE (caSLE group) and 10 healthy volunteers (control group) were enrolled in the study, in order to evaluate the VAP‑1 expression levels in blood. Protein expression profiling was performed with two‑dimensional fluorescence difference gel electrophoresis (2D‑DIGE) and analyzed with matrix‑assisted laser desorption/deionization time‑of‑flight (MALDI‑TOF) used in tandem with mass spectrometry. In the caSLE group, 8 proteins were expressed differenty compared with the control group: C‑reactive protein, hemoglobin subunit β, VAP‑1, A‑albumin (AFAM), enolase and immunoglobulin heavy constant mu were upregulated; interferon regulatory factor‑1 and serum amyloid A2 protein were downregulated. Western blotting results are consistent with the proteomics results, showing that in caSLE group VAP‑1 expression is increased in comparison to the control group. VAP‑1 protein participates in the inflammatory reaction in the form of amine oxidase, copper containing 3. Bioinformatics analysis suggested that VAP‑1 protein could be used as a candidate protein for further study regarding its role as a potential protein marker for screening and effectively monitoring caSLE.
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1 

Ferreira TA, de Andrade HM, de Pádua PM, Carvalho MD, Pires SD, Oliveira IH, Lima BS, Fialho Júnior LC, Cicarini WB, Chapeourouge DA, et al: Identification of potential biomarkers for systemic lupus erythematosus diagnosis using two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. Autoimmunity. 50:247–256. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Hung WT, Chen YM, Lan JL, Chen HH, Chen YH, Chen DY, Hsieh CW and Wen MC: Antinucleosome antibodies as a potential biomarker for the evaluation of renal pathological activity in patients with proliferative lupus nephritis. Lupus. 20:1404–1410. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Ravirajan CT, Rowse L, Mac Gowan JR and Isenberg DA: An analysis of clinical disease activity and nephritis-associated serum autoantibody profiles inpatients with systemic lupus erythematosus: A cross-sectional study. Rheumatology (Oxford). 40:1405–1412. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Förger F, Matthias T, Oppermann M, Becker H and Helmke K: Clinical significance of anti-ds DNA antibody isotypes: Ig G/Ig M ratio of anti-ds DNA antibodies as a prognostic marker for lupus nephritis. Lupus. 13:36–44. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Trendelenburg M, Lopez-Trascasa M, Potlukova E, Moll S, Regenass S, Frémeaux-Bacchi V, Martinez-Ara J, Jancova E, Picazo ML, Honsova E, et al: High prevalence of anti-C1q antibodies in biopsy-proven active lupus nephritis. Nephrol Dial Transplant. 21:3115–3121. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Xiang YJ and Dai SM: Prevalence of rheumatic diseases and disability in China. Rheumatol Int. 29:481–490. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Murata M, Noda K, Fukuhara J, Kanda A, Kase S, Saito W, Ozawa Y, Mochizuki S, Kimura S, Mashima Y, et al: Soluble vascular adhesion protein-1 accumulates in proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci. 53:4055–4062. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Solé M and Unzeta M: Vascular cell lines expressing SSAO/VAP-1: A new experimental tool to study its involvement in vascular diseases. Biol Cell. 103:543–557. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Feldman AS, Banyard J, Wu CL, McDougal WS and Zetter BR: Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression. Clin Cancer Res. 15:1024–1031. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Hotte NS and Deyholos MK: A flax fibre proteome: Identification of proteins enriched in bast fibres. BMC Plant Biol. 8:522008. View Article : Google Scholar : PubMed/NCBI

11 

Liu J, Huang P, He Y, Hong WX, Ren X, Yang X, He Y, Wang W, Zhang R, Yang H, et al: Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. PLoS One. 9:e1088162014. View Article : Google Scholar : PubMed/NCBI

12 

Junker H, Venz S, Zimmermann U, Thiele A, Scharf C and Walther R: Stage-related alterations in renal cell carcinoma-comprehensive quantitative analysis by 2D-DIGE and protein network analysis. PLoS One. 6:e218672011. View Article : Google Scholar : PubMed/NCBI

13 

Lappas M: Anti-inflammatory properties of sirtuin 6 in human umbilical vein endothelial cells. Mediators Inflamm. 2012:5975142012. View Article : Google Scholar : PubMed/NCBI

14 

Yuan H, Yao YS, Chen GM, Sheng J, Xu L and Pan HF: Decreased serum levels of T-cell immunoglobulin mucin-1 and T-cell immunoglobulin mucin-3 in systemic lupus erythematosus patients. J Biol Regul Homeost Agents. 30:123–129. 2016.PubMed/NCBI

15 

Penno MA, Klingler-Hoffmann M, Brazzatti JA, Boussioutas A, Putoczki T, Ernst M and Hoffmann P: 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer. J Proteomics. 77:402012. View Article : Google Scholar : PubMed/NCBI

16 

Zhen Y, Xu N, Richardson B, Becklin R, Savage JR, Blake K and Peltier JM: Development of an LC-MALDI method for the analysis of protein complexes. J Am Soc Mass Spectrom. 15:8032004. View Article : Google Scholar : PubMed/NCBI

17 

Deng L, Jia HL, Liu CW, Xu YF, Mao LJ, He CH, Yin GQ, Lin JH, Tao JP and Zhu L: Proteomic analysis of extremely severe hand, foot and mouth disease infected by enterovirus 71. BMC Infect Dis. 13:3832013. View Article : Google Scholar : PubMed/NCBI

18 

Wong MY, Saad S, Pollock CA and Wong MG: Semicarbazide-sensitive amine oxidase (SSAO) and kidney disease. Am J Physiol Renal Physiol. 305:1637–1644. 2013. View Article : Google Scholar

19 

Yu SL, Kuan WP, Wong CK, Li EK and Tam LS: Immunopathological roles of cytokines, chemokines, signaling molecules, and pattern-recognition receptors in systemic lupus erythematosus. Clin Dev Immunol. 2012:7151902012. View Article : Google Scholar : PubMed/NCBI

20 

Azkargorta M, Arizmendi JM, Elortza F, Alkorta N, Zubiaga AM and Fullaondo A: Differential proteome profiles in E2F2-deficient T lymphocytes. Proteomics. 6 (Suppl 1):S42–S50. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Dunkel J, Aguilar-Pimentel JA, Ollert M, Fuchs H, Gailus-Durner V, de Angelis MH, Jalkanen S, Salmi M and Veres TZ: Endothelial amine oxidase AOC3 transiently contributes to adaptive immune responses in the airways. Eur J Immunol. 44:3232–3239. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Kushimoto S, Shibata Y, Koido Y, Kawai M, Yokota H and Yamamoto Y: The clinical usefulness of procalcitonin measurement for assessing the severity of bacterial infection in critically ill patients requiring corticosteroid therapy. J Nippon Med Sch. 74:236–240. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Apostolidis SA, Lieberman LA, Kis-Toth K, Crispín JC and Tsokos GC: The dysregulation of cytokine networks in systemic lupus erythematosus. J Interferon Cytokine Res. 31:769–779. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Trivedi PJ, Tickle J, Vesterhus MN, Eddowes PJ, Bruns T, Vainio J, Parker R, Smith D, Liaskou E, Thorbjørnsen LW, et al: Vascular adhesion protein-1 is elevated in primary sclerosing cholangitis, is predictive of clinical outcome and facilitates recruitment of gut-tropic lymphocytes to liver in a substrate-dependent manner. Gut. 67:1135–1145. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Zhang J, Zhou P, Sun H, Katsarou M and Drakoulis N: Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Exp Ther Med 18: 5072-5077, 2019.
APA
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., & Drakoulis, N. (2019). Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Experimental and Therapeutic Medicine, 18, 5072-5077. https://doi.org/10.3892/etm.2019.8009
MLA
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., Drakoulis, N."Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE". Experimental and Therapeutic Medicine 18.6 (2019): 5072-5077.
Chicago
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., Drakoulis, N."Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE". Experimental and Therapeutic Medicine 18, no. 6 (2019): 5072-5077. https://doi.org/10.3892/etm.2019.8009
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Zhang J, Zhou P, Sun H, Katsarou M and Drakoulis N: Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Exp Ther Med 18: 5072-5077, 2019.
APA
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., & Drakoulis, N. (2019). Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE. Experimental and Therapeutic Medicine, 18, 5072-5077. https://doi.org/10.3892/etm.2019.8009
MLA
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., Drakoulis, N."Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE". Experimental and Therapeutic Medicine 18.6 (2019): 5072-5077.
Chicago
Liu, H., Zhang, J., Zhou, P., Sun, H., Katsarou, M., Drakoulis, N."Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE". Experimental and Therapeutic Medicine 18, no. 6 (2019): 5072-5077. https://doi.org/10.3892/etm.2019.8009
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