Open Access

Valsartan inhibits RhoA‑ROCK2‑MYL pathway in rat model of alcoholic cardiomyopathy

  • Authors:
    • Luyifei Li
    • Ling Jing
    • Jiyi Zhao
    • Jiachen Lv
    • Wen Yang
    • Weimin Li
    • Lijun Zhou
  • View Affiliations

  • Published online on: October 7, 2019     https://doi.org/10.3892/etm.2019.8079
  • Pages: 4313-4321
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate variations in the Ras homolog gene family, member A (RhoA)‑Rho‑associated protein kinase 2 (ROCK2)‑myosin light chain (MYL) pathway in a rat model of alcoholic cardiomyopathy (ACM) and the role of angiotensin‑converting enzyme inhibitor drugs. Rat models of ACM were established via alcoholic gavage + free access to alcohol. The structural and functional changes of the heart were analyzed by hematoxylin‑eosin staining, Masson's trichrome staining, immunohistochemistry staining, western blotting and fluorescence quantitative polymerase chain reaction. A total of 16 weeks later, a decreased ejection fraction and left ventricular fractional shortening in the alcohol group compared with the control group were demonstrated resulting in an increased left ventricular end diastolic diameter. These adverse effects were ameliorated following treatment with valsartan. In addition, the alcohol group revealed a disorganized arrangement of myocardial filaments, which was improved upon treatment with valsartan. RhoA and ROCK2 protein expression significantly increased in myocardial cells in the alcohol compared with the control group. Following drug intervention with valsartan, expression of RhoA and ROCK2 proteins were inhibited in the alcohol group. Furthermore, significantly elevated RhoA and ROCK2 and decreased MYL protein and mRNA expression in the alcohol group was demonstrated compared with the control group. Administration of valsartan reversed the expression profile of RhoA, ROCK and MYL in ACM. Expression of RhoA and ROCK were elevated with downregulation of MYL resulting in heart failure. However, the angiotensin receptor antagonist diminished the expression of RhoA and ROCK and enhanced the expression of MYL. The results of the present study suggest a curative effect of valsartan in ACM.
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December 2019
Volume 18 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Li, L., Jing, L., Zhao, J., Lv, J., Yang, W., Li, W., & Zhou, L. (2019). Valsartan inhibits RhoA‑ROCK2‑MYL pathway in rat model of alcoholic cardiomyopathy. Experimental and Therapeutic Medicine, 18, 4313-4321. https://doi.org/10.3892/etm.2019.8079
MLA
Li, L., Jing, L., Zhao, J., Lv, J., Yang, W., Li, W., Zhou, L."Valsartan inhibits RhoA‑ROCK2‑MYL pathway in rat model of alcoholic cardiomyopathy". Experimental and Therapeutic Medicine 18.6 (2019): 4313-4321.
Chicago
Li, L., Jing, L., Zhao, J., Lv, J., Yang, W., Li, W., Zhou, L."Valsartan inhibits RhoA‑ROCK2‑MYL pathway in rat model of alcoholic cardiomyopathy". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4313-4321. https://doi.org/10.3892/etm.2019.8079