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Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages

  • Authors:
    • Qiwen Shi
    • Lan Zhao
    • Leifang Zhang
  • View Affiliations / Copyright

    Affiliations: Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4295-4302
    |
    Published online on: October 7, 2019
       https://doi.org/10.3892/etm.2019.8084
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Abstract

Brucella infection is one of the most serious zoonoses worldwide, affecting humans and domestic and wild animals. Astragalus polysaccharide (APS) is extracted from astragalus, which exhibits bioactive properties, including immunomodulation and anti‑tumour and antiviral activity. The present study revealed that APS treatment promoted macrophage activation, the production of pro‑inflammatory cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin‑12 and interferon‑γ, and Brucella clearance in murine macrophages and spleens. APS treatment was also demonstrated to protect the integrity of macrophages during infection with live attenuated Brucella suis strain 2 (B. suis S2). The results from in vitro experiments were consistent with the findings from the in vivo study, showing the elevated secretion of TNF‑α and nitric oxide in APS‑treated murine peritoneal macrophages following B. suis S2 infection. The current study demonstrated the potential of APS in the control and treatment of Brucella infection, and the enhancement of host inflammatory and immune responses.
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1 

Yoon H, Moon OK, Lee SH, Lee WC, Her M, Jeong W, Jung SC and Kim DS: Epidemiology of brucellosis among cattle in Korea from 2001 to 2011. J Vet Sci. 15:537–543. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Reyes AW, Hop HT, Arayan LT, Huy TX, Min W, Lee HJ, Chang HH and Kim S: Nocodazole treatment interrupted Brucella abortus invasion in RAW 264.7 cells, and successfully attenuated splenic proliferation with enhanced inflammatory response in mice. Microb Pathog. 103:87–93. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Reyes AWB, Arayan LT, Hop HT, Ngoc Huy TX, Vu SH, Min W, Lee HJ and Kim S: Effects of gallic acid on signaling kinases in murine macrophages and immune modulation against Brucella abortus 544 infection in mice. Microb Pathog. 119:255–259. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Hou H, Liu X and Peng Q: The advances in brucellosis vaccines. Vaccine. 37:3981–3988. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Zhu L, Feng Y, Zhang G, Jiang H, Zhang Z, Wang N, Ding J and Suo X: Brucella suis strain 2 vaccine is safe and protective against heterologous Brucella spp. infections. Vaccine. 34:395–400. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Abbassi-Daloii T, Yousefi S, Sekhavati MH and Tahmoorespur M: Impact of heat shock protein 60KD in combination with outer membrane proteins on immune response against Brucella melitensis. APMIS. 126:65–75. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Barquero-Calvo E, Chaves-Olarte E, Weiss DS, Guzmán-Verri C, Chacón-Díaz C, Rucavado A, Moriyón I and Moreno E: Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection. PLoS One. 2:e6312007. View Article : Google Scholar : PubMed/NCBI

8 

Iwasaki A and Medzhitov R: Toll-like receptor control of the adaptive immune responses. Nat Immunol. 5:987–995. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Liu N, Wang L, Sun C, Yang L, Tang B, Sun W and Peng Q: Macrophage activation induced by Brucella DNA suppresses bacterial intracellular replication via enhancing NO production. Microb Pathog. 89:177–183. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Snyder GA, Deredge D, Waldhuber A, Fresquez T, Wilkins DZ, Smith PT, Durr S, Cirl C, Jiang J, Jennings W, et al: Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. J Biol Chem. 289:669–679. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Lalsiamthara J and Lee JH: Development and trial of vaccines against Brucella. J Vet Sci. 18:281–290. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Zhao Y, Hanniffy S, Arce-Gorvel V, Conde-Alvarez R, Oh S, Moriyón I, Mémet S and Gorvel JP: Immunomodulatory properties of Brucella melitensis lipopolysaccharide determinants on mouse dendritic cells in vitro and in vivo. Virulence. 9:465–479. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Luo X, Zhang X, Wu X, Yang X, Han C, Wang Z, Du Q, Zhao X, Liu SL, Tong D and Huang Y: Brucella downregulates tumor necrosis factor-α to promote intracellular survival via Omp25 regulation of different microRNAs in porcine and murine macrophages. Front Immunol. 8:20132018. View Article : Google Scholar : PubMed/NCBI

14 

Hop HT, Reyes AWB, Huy TXN, Arayan LT, Min W, Lee HJ, Rhee MH, Chang HH and Kim S: Activation of NF-kB-mediated TNF-induced antimicrobial immunity is required for the efficient brucella abortus clearance in RAW 264.7 cells. Front Cell Infect Microbiol. 7:4372017. View Article : Google Scholar : PubMed/NCBI

15 

Cui B, Liu W, Wang X, Chen Y, Du Q, Zhao X, Zhang H, Liu SL, Tong D and Huang Y: Brucella Omp25 upregulates miR-155, miR-21-5p, and miR-23b to inhibit interleukin-12 production via modulation of programmed Death-1 signaling in human monocyte/macrophages. Front Immunol. 8:7082017. View Article : Google Scholar : PubMed/NCBI

16 

Elfaki MG, Alaidan AA and Al-Hokail AA: Host response to Brucella infection: Review and future perspective. J Infect Dev Ctries. 9:697–701. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Sun B, Yu S, Zhao D, Guo S, Wang X and Zhao K: Polysaccharides as vaccine adjuvants. Vaccine. 36:5226–5234. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Chu X, Liu XJ, Qiu JM, Zeng XL, Bao HR and Shu J: Effects of Astragalus and Codonopsis pilosula polysaccharides on alveolar macrophage phagocytosis and inflammation in chronic obstructive pulmonary disease mice exposed to PM2.5. Environ Toxicol Pharmacol. 48:76–84. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Zhang W, Ma W, Zhang J, Song X, Sun W and Fan Y: The immunoregulatory activities of astragalus polysaccharide liposome on macrophages and dendritic cells. Int J Biol Macromol. 105:852–861. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Hou YC, Wu JM, Wang MY, Wu MH, Chen KY, Yeh SL and Lin MT: Modulatory effects of astragalus polysaccharides on T-cell polarization in mice with polymicrobial sepsis. Mediators Inflamm. 2015:8263192015. View Article : Google Scholar : PubMed/NCBI

21 

Zhang P, Liu X, Liu H, Wang W, Liu X, Li X and Wu X: Astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication. Microb Pathog. 114:124–128. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Zhou X, Liu Z, Long T, Zhou L and Bao Y: Immunomodulatory effects of herbal formula of astragalus polysaccharide (APS) and polysaccharopeptide (PSP) in mice with lung cancer. Int J Biol Macromol. 106:596–601. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Xu C, Shi Q, Zhang L and Zhao H: High molecular weight hyaluronan attenuates fine particulate matter-induced acute lung injury through inhibition of ROS-ASK1-p38/JNK-mediated epithelial apoptosis. Environ Toxicol Pharmacol. 59:190–198. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Snyder DT, Hedges JF and Jutila MA: Getting ‘Inside’ type I IFNs: Type I IFNs in intracellular bacterial infections. J Immunol Res. 2017:93618022017. View Article : Google Scholar : PubMed/NCBI

25 

Wang X, Li Z, Li B, Chi H, Li J, Fan H, Yao R, Li Q, Dong X, Chen M, et al: Bioluminescence imaging of colonization and clearance dynamics of brucella suis vaccine strain S2 in mice and guinea pigs. Mol Imaging Biol. 18:519–526. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Wei P, Lu Q, Cui G, Guan Z, Yang L, Sun C, Sun W and Peng Q: The role of TREM-2 in internalization and intracellular survival of Brucella abortus in murine macrophages. Vet Immunol Immunopathol. 163:194–201. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Watarai M, Makino S, Fujii Y, Okamoto K and Shirahata T: Modulation of Brucella-induced macropinocytosis by lipid rafts mediates intracellular replication. Cell Microbiol. 4:341–355. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Ahmed W, Zheng K and Liu ZF: Establishment of chronic infection: Brucella's stealth strategy. Front Cell Infect Microbiol. 6:302016. View Article : Google Scholar : PubMed/NCBI

29 

Dorneles EM, Teixeira-Carvalho A, Araújo MS, Sriranganathan N and Lage AP: Immune response triggered by Brucella abortus following infection or vaccination. Vaccine. 33:3659–3666. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Zhang K, Wang H, Guo F, Yuan L, Zhang W, Wang Y and Chen C: OMP31 of Brucella melitensis 16M impairs the apoptosis of macrophages triggered by TNF-α. Exp Ther Med. 12:2783–2789. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Barrionuevo P, Delpino MV, Pozner RG, Velásquez LN, Cassataro J and Giambartolomei GH: Brucella abortus induces intracellular retention of MHC-I molecules in human macrophages down-modulating cytotoxic CD8(+) T cell responses. Cell Microbiol. 15:487–502. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Salcedo SP, Marchesini MI, Lelouard H, Fugier E, Jolly G, Balor S, Muller A, Lapaque N, Demaria O, Alexopoulou L, et al: Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1. PLoS Pathog. 4:e212008. View Article : Google Scholar : PubMed/NCBI

33 

Billard E, Dornand J and Gross A: Brucella suis prevents human dendritic cell maturation and antigen presentation through regulation of tumor necrosis factor alpha secretion. Infect Immun. 75:4980–4989. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Zhou L, Liu Z, Wang Z, Yu S, Long T, Zhou X and Bao Y: Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo. Sci Rep. 7:448222017. View Article : Google Scholar : PubMed/NCBI

35 

Abdullahi AY, Kallon S, Yu X, Zhang Y and Li G: Vaccination with astragalus and ginseng polysaccharides improves immune response of chickens against H5N1 Avian influenza virus. Biomed Res Int. 2016:15102642016. View Article : Google Scholar : PubMed/NCBI

36 

Kallon S, Li X, Ji J, Chen C, Xi Q, Chang S, Xue C, Ma J, Xie Q and Zhang Y: Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo. J Anim Sci Biotechnol. 4:222013. View Article : Google Scholar : PubMed/NCBI

37 

Liu D, Su J, Lin J, Qian G, Chen X, Song S and Huang K: Activation of AMPK-dependent SIRT-1 by astragalus polysaccharide protects against ochratoxin A-induced immune stress in vitro and in vivo. Int J Biol Macromol. 120:683–692. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi Q, Zhao L and Zhang L: Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages. Exp Ther Med 18: 4295-4302, 2019.
APA
Shi, Q., Zhao, L., & Zhang, L. (2019). Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages. Experimental and Therapeutic Medicine, 18, 4295-4302. https://doi.org/10.3892/etm.2019.8084
MLA
Shi, Q., Zhao, L., Zhang, L."Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages". Experimental and Therapeutic Medicine 18.6 (2019): 4295-4302.
Chicago
Shi, Q., Zhao, L., Zhang, L."Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4295-4302. https://doi.org/10.3892/etm.2019.8084
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Q, Zhao L and Zhang L: Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages. Exp Ther Med 18: 4295-4302, 2019.
APA
Shi, Q., Zhao, L., & Zhang, L. (2019). Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages. Experimental and Therapeutic Medicine, 18, 4295-4302. https://doi.org/10.3892/etm.2019.8084
MLA
Shi, Q., Zhao, L., Zhang, L."Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages". Experimental and Therapeutic Medicine 18.6 (2019): 4295-4302.
Chicago
Shi, Q., Zhao, L., Zhang, L."Astragalus polysaccharide strengthens the inflammatory and immune responses of Brucella suis S2‑infected mice and macrophages". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4295-4302. https://doi.org/10.3892/etm.2019.8084
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