Open Access

Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer

  • Authors:
    • Qian Guo
    • Shuang Lv
    • Bingping Wang
    • Yinbing Li
    • Nier Cha
    • Ruigang Zhao
    • Wenhua Bao
    • Baoqing Jia
  • View Affiliations

  • Published online on: October 31, 2019     https://doi.org/10.3892/etm.2019.8152
  • Pages: 4547-4554
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) have important roles in the development and progression of various types of human cancer. However, the expression and function of the lncRNA prostate cancer‑associated non‑coding RNA 1 (PRNCR1) in breast cancer remains unclear. Reverse transcription‑quantitative PCR was performed to measure the levels of mRNA expression. Cell counting kit‑8, flow cytometry, wound healing and Transwell assays were also performed to study cell proliferation, cell cycle, migration and invasion, respectively. The results of the present study revealed that PRNCR1 expression levels were higher in breast cancer tissues compared with adjacent normal tissues in a patient study. It was also determined that high expression of PRNCR1 was significantly associated with advanced clinical stage, positive metastasis and poor prognosis for patients with breast cancer. In vitro experiments determined that PRNCR1 was significantly upregulated in the breast cancer cell lines BT‑549, MCF‑7, SK‑BR‑3 and MDA‑MB‑231 compared with the normal human breast cell line, MCF‑10A. Silencing of PRNCR1 significantly inhibited the proliferation, colony formation, cell cycle progression, migration and invasion of SK‑BR‑3 and BT‑549 cells, while cell apoptosis was induced. In addition, knockdown of PRNCR1 suppressed epithelial‑mesenchymal transition in SK‑BR‑3 and BT‑549 cells. In summary, the present results demonstrated that lncRNA PRNCR1 was significantly upregulated in breast cancer and was associated with cancer progression and poor patient prognosis. In vitro experiments determined that knockdown of PRNCR1 inhibited the malignant phenotypes of breast cancer cells. Taken together, the results indicated that PRNCR1 may be used as a potential therapeutic target for patients with breast cancer.

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December 2019
Volume 18 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Guo, Q., Lv, S., Wang, B., Li, Y., Cha, N., Zhao, R. ... Jia, B. (2019). Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer. Experimental and Therapeutic Medicine, 18, 4547-4554. https://doi.org/10.3892/etm.2019.8152
MLA
Guo, Q., Lv, S., Wang, B., Li, Y., Cha, N., Zhao, R., Bao, W., Jia, B."Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer". Experimental and Therapeutic Medicine 18.6 (2019): 4547-4554.
Chicago
Guo, Q., Lv, S., Wang, B., Li, Y., Cha, N., Zhao, R., Bao, W., Jia, B."Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4547-4554. https://doi.org/10.3892/etm.2019.8152