Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer

Guo,Qian; Lv,Shuang; Wang,Bingping; Li,Yinbing; Cha,Nier; Zhao,Ruigang; Bao,Wenhua; Jia,Baoqing

doi:10.3892/etm.2019.8152

Long non‑coding RNA PRNCR1 has an oncogenic role in breast cancer

Authors:
- Qian Guo
- Shuang Lv
- Bingping Wang
- Yinbing Li
- Nier Cha
- Ruigang Zhao
- Wenhua Bao
- Baoqing Jia
View Affiliations

Affiliations: Breast Neoplasms Surgical Department, Inner Mongolia People's Hospital, Huhehot, Inner Mongolia 010010, P.R. China, Department of Oncology, Inner Mongolia People's Hospital, Huhehot, Inner Mongolia 010010, P.R. China, Oncology Institute, Inner Mongolia People's Hospital, Huhehot, Inner Mongolia 010010, P.R. China
Published online on: October 31, 2019 https://doi.org/10.3892/etm.2019.8152
Pages: 4547-4554
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Long non‑coding RNAs (lncRNAs) have important roles in the development and progression of various types of human cancer. However, the expression and function of the lncRNA prostate cancer‑associated non‑coding RNA 1 (PRNCR1) in breast cancer remains unclear. Reverse transcription‑quantitative PCR was performed to measure the levels of mRNA expression. Cell counting kit‑8, flow cytometry, wound healing and Transwell assays were also performed to study cell proliferation, cell cycle, migration and invasion, respectively. The results of the present study revealed that PRNCR1 expression levels were higher in breast cancer tissues compared with adjacent normal tissues in a patient study. It was also determined that high expression of PRNCR1 was significantly associated with advanced clinical stage, positive metastasis and poor prognosis for patients with breast cancer. In vitro experiments determined that PRNCR1 was significantly upregulated in the breast cancer cell lines BT‑549, MCF‑7, SK‑BR‑3 and MDA‑MB‑231 compared with the normal human breast cell line, MCF‑10A. Silencing of PRNCR1 significantly inhibited the proliferation, colony formation, cell cycle progression, migration and invasion of SK‑BR‑3 and BT‑549 cells, while cell apoptosis was induced. In addition, knockdown of PRNCR1 suppressed epithelial‑mesenchymal transition in SK‑BR‑3 and BT‑549 cells. In summary, the present results demonstrated that lncRNA PRNCR1 was significantly upregulated in breast cancer and was associated with cancer progression and poor patient prognosis. In vitro experiments determined that knockdown of PRNCR1 inhibited the malignant phenotypes of breast cancer cells. Taken together, the results indicated that PRNCR1 may be used as a potential therapeutic target for patients with breast cancer.

View Figures

View References

1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI
2	Vishal M, Swetha R, Thejaswini G, Arumugam B and Selvamurugan N: Role of Runx2 in breast cancer-mediated bone metastasis. Int J Biol Macromol. 99:608–614. 2017. View Article : Google Scholar : PubMed/NCBI
3	Xu S, Kong D, Chen Q, Ping Y and Pang D: Oncogenic long noncoding RNA landscape in breast cancer. Mol Cancer. 16:1292017. View Article : Google Scholar : PubMed/NCBI
4	Luo T, Yan Y, He Q, Ma X and Wang W: miR-328-5p inhibits MDA-MB-231 breast cancer cell proliferation by targeting RAGE. Oncol Rep. 39:2906–2914. 2018.PubMed/NCBI
5	Zhou Y, Meng X, Chen S, Li W, Li D, Singer R and Gu W: IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p. Breast Cancer Res. 20:322018. View Article : Google Scholar : PubMed/NCBI
6	Peng Z, Liu C and Wu M: New insights into long noncoding RNAs and their roles in glioma. Mol Cancer. 17:612018. View Article : Google Scholar : PubMed/NCBI
7	Taylor DH, Chu ET, Spektor R and Soloway PD: Long non-coding RNA regulation of reproduction and development. Mol Reprod Dev. 82:932–956. 2015. View Article : Google Scholar : PubMed/NCBI
8	Wang O, Yang F, Liu Y, Lv L, Ma R, Chen C, Wang J, Tan Q, Cheng Y, Xia E, et al: C-MYC-induced upregulation of lncRNA SNHG12 regulates cell proliferation, apoptosis and migration in triple-negative breast cancer. Am J Transl Res. 9:533–545. 2017.PubMed/NCBI
9	Guan YX, Zhang ZM, Chen XZ, Zhang Q, Liu SZ and Zhang YL: Lnc RNA SNHG20 participated in proliferation, invasion and migration of breast cancer cells via miR-495. J Cell Biochem. 119:7971–7981. 2018. View Article : Google Scholar : PubMed/NCBI
10	Jiang X, Zhou Y, Sun AJ and Xue JL: NEAT1 contributes to breast cancer progression through modulating miR-448 and ZEB1. J Cell Physiol. 233:8558–8566. 2018. View Article : Google Scholar : PubMed/NCBI
11	Li W, Zhai L, Wang H, Liu C, Zhang J, Chen W and Wei Q: Downregulation of LncRNA GAS5 causes trastuzumab resistance in breast cancer. Oncotarget. 7:27778–27786. 2016.PubMed/NCBI
12	Chung S, Nakagawa H, Uemura M, Piao L, Ashikawa K, Hosono N, Takata R, Akamatsu S, Kawaguchi T, Morizono T, et al: Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility. Cancer Sci. 102:245–252. 2011. View Article : Google Scholar : PubMed/NCBI
13	Li L, Sun R, Liang Y, Pan X, Li Z, Bai P, Zeng X, Zhang D, Zhang L and Gao L: Association between polymorphisms in long non-coding RNA PRNCR1 in 8q24 and risk of colorectal cancer. J Exp Clin Cancer Res. 32:1042013. View Article : Google Scholar : PubMed/NCBI
14	Pestell RG and Yu Z: Long and noncoding RNAs (lnc-RNAs) determine androgen receptor dependent gene expression in prostate cancer growth in vivo. Asian J Androl. 16:268–269. 2014. View Article : Google Scholar : PubMed/NCBI
15	Yang L, Qiu M, Xu Y, Wang J, Zheng Y, Li M, Xu L and Yin R: Upregulation of long non-coding RNA PRNCR1 in colorectal cancer promotes cell proliferation and cell cycle progression. Oncol Rep. 35:318–324. 2016. View Article : Google Scholar : PubMed/NCBI
16	Cheng D, Bao C, Zhang X, Lin X, Huang H and Zhao L: LncRNA PRNCR1 interacts with HEY2 to abolish miR-448-mediated growth inhibition in non-small cell lung cancer. Biomed Pharmacother. 107:1540–1547. 2018. View Article : Google Scholar : PubMed/NCBI
17	Gong ZM, Tang ZY and Sun XL: LncRNA PRNCR1 regulates CXCR4 expression to affect osteogenic differentiation and contribute to osteolysis after hip replacement. Gene. 673:251–261. 2018. View Article : Google Scholar : PubMed/NCBI
18	Jiao S, Wang SY and Huang Y: LncRNA PRNCR1 promoted the progression of eclampsia by regulating the MAPK signal pathway. Eur Rev Med Pharmacol Sci. 22:3635–3642. 2018.PubMed/NCBI
19	Liu MC, Pitcher BN, Mardis ER, Davies SR, Friedman PN, Snider JE, Vickery TL, Reed JP, DeSchryver K, Singh B, et al: PAM50 gene signatures and breast cancer prognosis with adjuvant anthracycline- and taxane-based chemotherapy: Correlative analysis of C9741 (Alliance). NPJ Breast Cancer. 2:e150232016. View Article : Google Scholar
20	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
21	Li C, Wan L, Liu Z, Xu G, Wang S, Su Z, Zhang Y, Zhang C, Liu X, Lei Z and Zhang HT: Long non-coding RNA XIST promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-367/141-ZEB2 axis in non-small-cell lung cancer. Cancer Lett. 418:185–195. 2018. View Article : Google Scholar : PubMed/NCBI
22	Sun W, Li AQ, Zhou P, Jiang YZ, Jin X, Liu YR, Guo YJ, Yang WT, Shao ZM and Xu XE: DSCAM-AS1 regulates the G1/S cell cycle transition and is an independent prognostic factor of poor survival in luminal breast cancer patients treated with endocrine therapy. Cancer Med. 7:6137–6146. 2018. View Article : Google Scholar : PubMed/NCBI
23	Hu X, Ding D, Zhang J and Cui J: Knockdown lncRNA HOTAIR sensitize breast cancer cells to ionizing radiation through activating miR-218. Biosci Rep. 39:BSR201810382019. View Article : Google Scholar : PubMed/NCBI
24	Qiao E, Chen D, Li Q, Feng W, Yu X, Zhang X, Xia L, Jin J and Yang H: Long noncoding RNA TALNEC2 plays an oncogenic role in breast cancer by binding to EZH2 to target p57(KIP2) and involving in p-p38 MAPK and NF-κB pathways. J Cell Biochem. 120:3978–3988. 2019. View Article : Google Scholar : PubMed/NCBI
25	Smith BN and Bhowmick NA: Role of EMT in metastasis and therapy resistance. J Clin Med. 5:E172016. View Article : Google Scholar : PubMed/NCBI
26	Xia XL, Xue D, Xiang TH, Xu HY, Song DK, Cheng PG and Wang JQ: Overexpression of long non-coding RNA CRNDE facilitates epithelial-mesenchymal transition and correlates with poor prognosis in intrahepatic cholangiocarcinoma. Oncol Lett. 15:4105–4112. 2018.PubMed/NCBI