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Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer

  • Authors:
    • Jing Zhang
    • Jun‑Rong Lei
    • Ling‑Ling Yuan
    • Ru Wen
    • Jiong Yang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Chemistry, University of Georgia, Athens, GA 30602, USA
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 107-114
    |
    Published online on: November 8, 2019
       https://doi.org/10.3892/etm.2019.8177
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Abstract

Response gene to complement (RGC)‑32 regulates the cell cycle in response to complement activation. The present study demonstrated that the expression level of RGC‑32 is higher in human non‑small‑cell lung cancer (NSCLC) tissues compared with health controls. Overexpressing RGC‑32 induced p65 nucleus translocation, significantly increased nuclear p65 levels and promoted the proliferation of A549 cells. Knockdown of RGC‑32 by short hairpin RNA decreased the expression level of nuclear p65 and inhibited cell proliferation. The increase in cell proliferation induced by RGC32 could be abolished by the NF‑κB inhibitor pyrrolidine dithiocarbamate. Mechanistic studies indicated that RGC32 mediated NF‑κB downstream genes, including vascular cell adhesion protein 1, interleukin‑6, cyclin dependent kinase inhibitor 2C, testin and vascular endothelial growth factor A. In summary, the present study demonstrated a novel role of RGC‑32 in the progression of NSCLC via the NF‑κB pathway and p65. Therefore, RGC‑32 could be a potential therapeutic target for NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Lei JR, Yuan LL, Wen R and Yang J: Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer. Exp Ther Med 19: 107-114, 2020.
APA
Zhang, J., Lei, J., Yuan, L., Wen, R., & Yang, J. (2020). Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer. Experimental and Therapeutic Medicine, 19, 107-114. https://doi.org/10.3892/etm.2019.8177
MLA
Zhang, J., Lei, J., Yuan, L., Wen, R., Yang, J."Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer". Experimental and Therapeutic Medicine 19.1 (2020): 107-114.
Chicago
Zhang, J., Lei, J., Yuan, L., Wen, R., Yang, J."Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer". Experimental and Therapeutic Medicine 19, no. 1 (2020): 107-114. https://doi.org/10.3892/etm.2019.8177
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Lei JR, Yuan LL, Wen R and Yang J: Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer. Exp Ther Med 19: 107-114, 2020.
APA
Zhang, J., Lei, J., Yuan, L., Wen, R., & Yang, J. (2020). Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer. Experimental and Therapeutic Medicine, 19, 107-114. https://doi.org/10.3892/etm.2019.8177
MLA
Zhang, J., Lei, J., Yuan, L., Wen, R., Yang, J."Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer". Experimental and Therapeutic Medicine 19.1 (2020): 107-114.
Chicago
Zhang, J., Lei, J., Yuan, L., Wen, R., Yang, J."Response gene to complement‑32 promotes cell survival via the NF‑κB pathway in non‑small‑cell lung cancer". Experimental and Therapeutic Medicine 19, no. 1 (2020): 107-114. https://doi.org/10.3892/etm.2019.8177
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