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Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats

  • Authors:
    • Bing Li
    • Xiaoyan Xiao
    • Yulian Miao
    • Ling Guo
    • Junhui Zhen
    • Xianhua Li
    • Bei Jiang
    • Zhao Hu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Medical Administration, Weifang Traditional Chinese Medicine Hospital, Weifang, Shandong 261041, P.R. China, Department of Pathology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 123-130
    |
    Published online on: November 8, 2019
       https://doi.org/10.3892/etm.2019.8178
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Abstract

Obesity‑associated podocyte injury increases in parallel with inflammatory responses. Resveratrol, as an anti‑inflammatory and antioxidative agent, has been proven to be effective in obesity. The purpose of the present study was to investigate the function of resveratrol in the early stages of podocyte injury in ovariectomized rats fed a high‑fat diet (HFD). The 3‑month‑old female Wistar rats were randomly divided into four groups: Sham operation with a standard diet; sham operation with a HFD; ovariectomy with a HFD (O+H); and ovariectomy plus HFD treatment with resveratrol (40 mg/kg/day) (O+H+R). Following 12 weeks, the weights of the rats were measured and serum was obtained to measure the levels of 17β‑estradiol (E2), serum lipids, serum creatinine, fasting blood glucose (FBG) and insulin. Periodic acid‑schiff staining was used to detect renal pathological changes. Meanwhile, the expression of the podocyte‑associated proteins nephrin and Wilms' tumor‑1 was investigated using immunohistochemical staining, and the levels of tumor necrosis factor‑α, interleukin‑6 and monocyte chemotactic protein‑1 in renal tissues were determined using western blotting. Compared with the O+H group, resveratrol significantly reduced the body weight, serum levels of total cholesterol, low‑density lipoprotein cholesterol, FBG and insulin resistance, and increased the levels of E2 in obese rats with an ovariectomy combined with an HFD (P<0.05). Compared with the O+H group, although the O+H+R group had no significant changes in renal pathology, the changes in the levels of podocyte‑associated proteins and inflammatory markers were significantly reversed (P<0.05). These results suggest that resveratrol may attenuate early podocyte injury by improving lipid metabolism and insulin sensitivity, and subsequently inhibiting inflammatory responses in obese rats induced by an ovariectomy with a HFD.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Xiao X, Miao Y, Guo L, Zhen J, Li X, Jiang B and Hu Z: Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats. Exp Ther Med 19: 123-130, 2020.
APA
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X. ... Hu, Z. (2020). Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats. Experimental and Therapeutic Medicine, 19, 123-130. https://doi.org/10.3892/etm.2019.8178
MLA
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X., Jiang, B., Hu, Z."Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats". Experimental and Therapeutic Medicine 19.1 (2020): 123-130.
Chicago
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X., Jiang, B., Hu, Z."Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats". Experimental and Therapeutic Medicine 19, no. 1 (2020): 123-130. https://doi.org/10.3892/etm.2019.8178
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Xiao X, Miao Y, Guo L, Zhen J, Li X, Jiang B and Hu Z: Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats. Exp Ther Med 19: 123-130, 2020.
APA
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X. ... Hu, Z. (2020). Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats. Experimental and Therapeutic Medicine, 19, 123-130. https://doi.org/10.3892/etm.2019.8178
MLA
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X., Jiang, B., Hu, Z."Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats". Experimental and Therapeutic Medicine 19.1 (2020): 123-130.
Chicago
Li, B., Xiao, X., Miao, Y., Guo, L., Zhen, J., Li, X., Jiang, B., Hu, Z."Resveratrol alleviates obesity‑associated podocyte injury in ovariectomized obese rats". Experimental and Therapeutic Medicine 19, no. 1 (2020): 123-130. https://doi.org/10.3892/etm.2019.8178
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