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Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis

  • Authors:
    • Jialin Zhang
    • Bin Fu
    • Xiaolei Chen
    • Desheng Chen
    • Hao Yang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, General Hospital of Ningxia Medical University, Ningxia, Gansu 750004, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 232-240
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    Published online on: November 12, 2019
       https://doi.org/10.3892/etm.2019.8189
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Abstract

Osteoarthritis (OA) is related to over‑proliferation or differentiation of osteoclasts. Although protocatechuic acid (PCA) has been identified to inhibit osteoclast differentiation and stimulate apoptosis in mature osteoclasts, whether it can relieve OA is still unknown. The present study aimed to investigate the effect of PCA on anterior cruciate ligament transection (ACLT)‑induced OA and the potential mechanisms of action behind this effect. ACLT was performed on rats, which were then treated with or without PCA. C‑terminal telopeptide of type I collagen (CTX‑I) and CTX‑II were tested in knee joint protein extracts by ELISA. Damage to cartilage was evaluated using Safranin‑O/Fast Green staining. Osteoclast‑related gene and protein expression was assessed through reverse transcription‑quantitative PCR and western blotting. Tartrate‑resistant acid phosphatase (TRAP) staining and functional bone resorption pit assays were performed using RAW264.7 murine macrophage cells to determine the effects of PCA on osteoclastic formation and function, respectively, in vitro. Finally, the activity of osteoclastogenesis‑related signaling pathways was evaluated by western blotting. Levels of CTX‑II were relatively decreased and Safranin‑O/fast green staining indicated milder changes in the articular cartilage in the PCA treatment group. PCA downregulated osteoclast specific markers and suppressed receptor activator of nuclear factor‑κB ligand‑induced formation of TRAP‑positive multinucleated cells, bone‑resorption and pit formation. Mitogen‑activated protein kinase (MAPK) and Akt signaling as well as the downstream factors, were downregulated by PCA. In conclusion, the present study demonstrated that PCA attenuated ACLT‑induced OA by suppressing osteoclastogenesis by inhibiting the MAPK, ATK and NF‑κB signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Fu B, Chen X, Chen D and Yang H: Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis. Exp Ther Med 19: 232-240, 2020.
APA
Zhang, J., Fu, B., Chen, X., Chen, D., & Yang, H. (2020). Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis. Experimental and Therapeutic Medicine, 19, 232-240. https://doi.org/10.3892/etm.2019.8189
MLA
Zhang, J., Fu, B., Chen, X., Chen, D., Yang, H."Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis". Experimental and Therapeutic Medicine 19.1 (2020): 232-240.
Chicago
Zhang, J., Fu, B., Chen, X., Chen, D., Yang, H."Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis". Experimental and Therapeutic Medicine 19, no. 1 (2020): 232-240. https://doi.org/10.3892/etm.2019.8189
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Fu B, Chen X, Chen D and Yang H: Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis. Exp Ther Med 19: 232-240, 2020.
APA
Zhang, J., Fu, B., Chen, X., Chen, D., & Yang, H. (2020). Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis. Experimental and Therapeutic Medicine, 19, 232-240. https://doi.org/10.3892/etm.2019.8189
MLA
Zhang, J., Fu, B., Chen, X., Chen, D., Yang, H."Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis". Experimental and Therapeutic Medicine 19.1 (2020): 232-240.
Chicago
Zhang, J., Fu, B., Chen, X., Chen, D., Yang, H."Protocatechuic acid attenuates anterior cruciate ligament transection‑induced osteoarthritis by suppressing osteoclastogenesis". Experimental and Therapeutic Medicine 19, no. 1 (2020): 232-240. https://doi.org/10.3892/etm.2019.8189
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