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Article Open Access

Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B

Retraction in: /10.3892/etm.2025.12875
  • Authors:
    • Yinong Yang
    • Qing Li
    • Feng Tan
    • Jun Zhang
    • Wu Zhu
  • View Affiliations / Copyright

    Affiliations: Medical School, Hunan University of Medicine, Huaihua, Hunan 418000, P.R. China, Nursing School, Hunan University of Medicine, Huaihua, Hunan 418000, P.R. China, School of Stomatology, Hunan University of Medicine, Huaihua, Hunan 418000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 287-293
    |
    Published online on: November 13, 2019
       https://doi.org/10.3892/etm.2019.8192
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Abstract

This study explored how interleukin‑8 (IL‑8) causes acute lung injury (ALI) through pulmonary surfactant protein A (SP‑A) and surfactant protein B (SP‑B). Serum was collected from 53 ALI patients and further 56 healthy subjects who underwent physical examination. The IL‑8, SP‑A, and SP‑B levels were determined using enzyme‑linked immunosorbent assay (ELISA). An ALI model was constructed using lipopolysaccharide (LSP)‑induced normal A549 cells. siRNA was employed to interfere with the expression of IL‑8, SP‑A and SP‑B. Western blot analysis was carried out to determine the protein levels, and MTT assay to determine the cell activity. In addition, co‑immunoprecipitation (Co‑IP) assay was used to verify the interaction between IL‑8, SP‑A and SP‑B. ALI patients showed high expression of serum IL‑8, and low expression of SP‑A and SP‑B, and IL‑8 was negatively correlated with SP‑A and SP‑B, respectively. LSP‑induced normal A549 cells showed increased expression of IL‑8 and decreased expression of SP‑A and SP‑B. Silencing IL‑8 led to increased expression levels of SP‑A, SP‑B and Bcl2, decreased expression levels of caspase‑9, caspase‑3, Bax, TNF‑α, IL‑17 and IL‑1β, reduced cell apoptosis rate, and enhanced cell viability. Silencing SP‑A and SP‑B resulted in increased expression of IL‑8, caspase‑9, caspase‑3, Bax, TNF‑α, IL‑17 and IL‑1β, and decreased expression of Bcl2. Co‑IP assay revealed that IL‑8 could interact with SP‑A and SP‑B, respectively. IL‑8 induces apoptosis by inhibiting SP‑A and SP‑B, and intensifies cellular inflammatory reaction, leading eventually to ALI.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Li Q, Tan F, Zhang J and Zhu W: Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875. Exp Ther Med 19: 287-293, 2020.
APA
Yang, Y., Li, Q., Tan, F., Zhang, J., & Zhu, W. (2020). Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875. Experimental and Therapeutic Medicine, 19, 287-293. https://doi.org/10.3892/etm.2019.8192
MLA
Yang, Y., Li, Q., Tan, F., Zhang, J., Zhu, W."Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875". Experimental and Therapeutic Medicine 19.1 (2020): 287-293.
Chicago
Yang, Y., Li, Q., Tan, F., Zhang, J., Zhu, W."Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875". Experimental and Therapeutic Medicine 19, no. 1 (2020): 287-293. https://doi.org/10.3892/etm.2019.8192
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Li Q, Tan F, Zhang J and Zhu W: Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875. Exp Ther Med 19: 287-293, 2020.
APA
Yang, Y., Li, Q., Tan, F., Zhang, J., & Zhu, W. (2020). Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875. Experimental and Therapeutic Medicine, 19, 287-293. https://doi.org/10.3892/etm.2019.8192
MLA
Yang, Y., Li, Q., Tan, F., Zhang, J., Zhu, W."Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875". Experimental and Therapeutic Medicine 19.1 (2020): 287-293.
Chicago
Yang, Y., Li, Q., Tan, F., Zhang, J., Zhu, W."Mechanism of IL‑8‑induced acute lung injury through pulmonary surfactant proteins A and B Retraction in /10.3892/etm.2025.12875". Experimental and Therapeutic Medicine 19, no. 1 (2020): 287-293. https://doi.org/10.3892/etm.2019.8192
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