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Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress

  • Authors:
    • Nipin Sp
    • Dong Young Kang
    • Do Hoon Kim
    • Hyo Gun Lee
    • Yeong‑Min Park
    • Il Ho Kim
    • Hak Kyo Lee
    • Byung‑Wook Cho
    • Kyoung‑Jin Jang
    • Young Mok Yang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju, Chungcheongbuk 27478, Republic of Korea, Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang, Gyeongsangnam 50463, Republic of Korea, Department of Immunology, School of Medicine, Konkuk University, Chungju, Chungcheongbuk 27478, Republic of Korea, Nara Biotech Co., Ltd., Jeonju, Jeollabuk 54852, Republic of Korea, Department of Animal Biotechnology, Chonbuk National University, Jeonju, Jeollabuk 54896, Republic of Korea
    Copyright: © Sp et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 214-222
    |
    Published online on: November 13, 2019
       https://doi.org/10.3892/etm.2019.8196
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Abstract

Cortisol is a hormone involved in stress during exercise. The application of natural compounds is a new potential approach for controlling cortisol‑induced stress. Tumour suppressor protein p53 is activated during cellular stress. Succinate dehydrogenase complex subunit A (SDHA) and hypoxanthine phosphoribosyl transferase 1 (HPRT1) are considered to be two of the most stable reference genes when measuring stress during exercise in horses. In the present study cells were considered to be in a ʻstressed stateʼ if the levels of these stable genes and the highly stress responsive gene p53 were altered. It was hypothesized that a natural organic sulphur‑containing compound, methylsulfonylmethane (MSM), could inhibit cortisol‑induced stress in racing horse skeletal muscle cells by regulating SDHA, HPRT1 and p53 expression. After assessing cell viability using MTT assays, 20 µg/ml cortisol and 50 mM MSM were applied to horse skeletal muscle cell cultures. Reverse transcription‑quantitative PCR and western blot analysis demonstrated increases in SDHA, HPRT1 and p53 expression in cells in response to cortisol treatment, which was inhibited or normalized by MSM treatment. To determine the relationship between p53 and SDHA/HPRT1 expression at a transcriptional level, horse gene sequences of SDHA and HPRT1 were probed to identify novel binding sites for p53 in the gene promoters, which were confirmed using a chromatin immunoprecipitation assay. The relationship between p53 and SDHA/HPRT1 expression was confirmed using western blot analysis following the application of pifithrin‑α, a p53 inhibitor. These results suggested that MSM is a potential candidate drug for the inhibition of cortisol‑induced stress in racehorse skeletal muscle cells.
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Copy and paste a formatted citation
Spandidos Publications style
Sp N, Kang DY, Kim DH, Lee HG, Park YM, Kim IH, Lee HK, Cho BW, Jang KJ, Yang YM, Yang YM, et al: Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress. Exp Ther Med 19: 214-222, 2020.
APA
Sp, N., Kang, D.Y., Kim, D.H., Lee, H.G., Park, Y., Kim, I.H. ... Yang, Y.M. (2020). Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress. Experimental and Therapeutic Medicine, 19, 214-222. https://doi.org/10.3892/etm.2019.8196
MLA
Sp, N., Kang, D. Y., Kim, D. H., Lee, H. G., Park, Y., Kim, I. H., Lee, H. K., Cho, B., Jang, K., Yang, Y. M."Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress". Experimental and Therapeutic Medicine 19.1 (2020): 214-222.
Chicago
Sp, N., Kang, D. Y., Kim, D. H., Lee, H. G., Park, Y., Kim, I. H., Lee, H. K., Cho, B., Jang, K., Yang, Y. M."Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress". Experimental and Therapeutic Medicine 19, no. 1 (2020): 214-222. https://doi.org/10.3892/etm.2019.8196
Copy and paste a formatted citation
x
Spandidos Publications style
Sp N, Kang DY, Kim DH, Lee HG, Park YM, Kim IH, Lee HK, Cho BW, Jang KJ, Yang YM, Yang YM, et al: Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress. Exp Ther Med 19: 214-222, 2020.
APA
Sp, N., Kang, D.Y., Kim, D.H., Lee, H.G., Park, Y., Kim, I.H. ... Yang, Y.M. (2020). Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress. Experimental and Therapeutic Medicine, 19, 214-222. https://doi.org/10.3892/etm.2019.8196
MLA
Sp, N., Kang, D. Y., Kim, D. H., Lee, H. G., Park, Y., Kim, I. H., Lee, H. K., Cho, B., Jang, K., Yang, Y. M."Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress". Experimental and Therapeutic Medicine 19.1 (2020): 214-222.
Chicago
Sp, N., Kang, D. Y., Kim, D. H., Lee, H. G., Park, Y., Kim, I. H., Lee, H. K., Cho, B., Jang, K., Yang, Y. M."Methylsulfonylmethane inhibits cortisol‑induced stress through p53‑mediated SDHA/HPRT1 expression in racehorse skeletal muscle cells: A primary step against exercise stress". Experimental and Therapeutic Medicine 19, no. 1 (2020): 214-222. https://doi.org/10.3892/etm.2019.8196
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