Open Access

Increase in liver γδ T cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection

  • Authors:
    • Lin Chang
    • Lei Wang
    • Ning Ling
    • Hui Peng
    • Min Chen
  • View Affiliations

  • Published online on: November 13, 2019     https://doi.org/10.3892/etm.2019.8197
  • Pages: 67-78
  • Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The role of γδ T cells in acute hepatitis B virus (HBV) infection remains unclear. For the present study, a mouse model of acute HBV infection was constructed using hydrodynamic injection‑based transfection of an HBV DNA plasmid (pHBV). Subsequent changes in the percentages of γδ T cells, expression of activation molecules (CD25 and CD69) and the production of the inflammatory cytokines interferon (IFN)‑γ and tumor necrosis factor‑α (TNF‑α) by liver γδ T cells were investigated using fluorescence‑activated cell sorting (FACS). Additionally, the immune responses in the mouse liver were evaluated dynamically by measuring cytokine mRNA expression (IFN‑α, IFN‑β, IFN‑γ or TNF‑α) using reverse transcription‑quantitative PCR, and other populations of immune cells, including CD4+T, CD8+T, natural killer (NK) or natural killer T (NKT) cells, using FACS. On day 1 following acute HBV infection, the percentage of liver γδ T cells was significantly increased along with the high expression of HBV markers. Additionally, liver γδ T cells displayed peak expression of the activation marker CD69 and peak IFN‑γ production within this timeframe. IFN‑β mRNA expression and the percentage of NK cells were elevated significantly on day 1 in liver tissues. However, there were no significant changes in the spleen or peripheral γδ T cells. Therefore, these data suggested that during the early stages of acute HBV infection, significantly increased numbers of liver γδ T cells may be involved in the enhanced immune response to the increased expression of HBV markers in the liver.
View Figures
View References

Related Articles

Journal Cover

January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chang L, Wang L, Ling N, Peng H and Chen M: Increase in liver γδ T cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection. Exp Ther Med 19: 67-78, 2020
APA
Chang, L., Wang, L., Ling, N., Peng, H., & Chen, M. (2020). Increase in liver γδ T cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection. Experimental and Therapeutic Medicine, 19, 67-78. https://doi.org/10.3892/etm.2019.8197
MLA
Chang, L., Wang, L., Ling, N., Peng, H., Chen, M."Increase in liver γδ T cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection". Experimental and Therapeutic Medicine 19.1 (2020): 67-78.
Chicago
Chang, L., Wang, L., Ling, N., Peng, H., Chen, M."Increase in liver γδ T cells with concurrent augmentation of IFN‑β production during the early stages of a mouse model of acute experimental hepatitis B virus infection". Experimental and Therapeutic Medicine 19, no. 1 (2020): 67-78. https://doi.org/10.3892/etm.2019.8197