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LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer

  • Authors:
    • Shengli Shi
    • Defeng Li
    • Yingfei Li
    • Zhiqiang Feng
    • Yanlei Du
    • Yuqiang Nie
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou Key Laboratory of Digestive Disease, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Gastroenterology, The 2nd Clinical Medicine College (Shenzhen People's Hospital) of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 569-578
    |
    Published online on: November 21, 2019
       https://doi.org/10.3892/etm.2019.8226
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Abstract

Long non‑coding RNAs (lncRNAs) are novel regulators for post‑transcriptional gene expression, and altered lncRNAs function and expression are associated with tumorigenesis and cancer progression, although the biological functions of most lncRNAs in various cancer types and their underlying regulatory interactions have remained largely elusive. Our previous study identified microRNA (miR)‑181a as a regulator of Kruppel‑like factor 6 (KLF6). In the present study, a bioinformatical analysis was performed to identify the novel lncRNA CR749391 as a potential regulator of miR‑181a that contains four putative binding sites. Subsequent in vitro experiments in gastric cancer (GC) cells demonstrated that CR749391 interacted with miR‑181a to regulate KLF6 expression. First, a direct binding interaction was confirmed using luciferase reporter and RNA immunoprecipitation and pull‑down assays. In addition, CR749391 was observed to be downregulated in GC compared with that of normal gastric cell lines. A functional study also revealed that CR749391 depletion in normal gastric epithelial cells promoted cell viability, migration and invasion, and conferred resistance to apoptosis, whereas ectopic CR749391 overexpression had the opposite effect in GC cells and inhibited in vivo tumor growth. In addition, CR749391 was observed to be downregulated in GC compared with that of normal gastric tissues, which was associated with KLF6 but inversely associated with miR‑181a levels. Overall, the CR749391/miR‑181a regulatory interaction and association between CR749391 and KLF6 may enhance the current understanding of GC pathogenesis, although CR749391 association with GC prognosis needs further study. The current study could provide a novel approach for lncRNA‑mediated targeted GC therapy.
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1 

Liu XH, Sun M, Nie FQ, Ge YB, Zhang EB, Yin DD, Kong R, Xia R, Lu KH, Li JH, et al: Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer. Mol Cancer. 13:922014. View Article : Google Scholar : PubMed/NCBI

2 

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA and Jema A: Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 68:394–424. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Miller KD, Nogueira L, Mariotto AB, Rowland JH, Yabroff KR, Alfano CM, Jemal A, Kramer JL and Siegel RL: Cancer treatment and survivorship statistics, 2019. CA Cancer J Clin. 69:363–385. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Yang L: Incidence and mortality of gastric cancer in China. World J Gastroenterol. 12:17–20. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Crew KD and Neugut AI: Epidemiology of gastric cancer. World J Gastroenterol. 12:354–362. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Hartgrink HH, Jansen EP, van Grieken NC and van de Velde CJ: Gastric cancer. Lancet. 374:477–490. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Ueda T, Volinia S, Okumura H, Shimizu M, Taccioli C, Rossi S, Alder H, Liu CG, Oue N, Yasui W, et al: Relation between microRNA expression and progression and prognosis of gastric cancer: A microRNA expression analysis. Lancet Oncol. 11:136–146. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Ponting CP, Oliver PL and Reik W: Evolution and functions of long noncoding RNAs. Cell. 136:629–641. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Arita T, Ichikawa D, Konishi H, Komatsu S, Shiozaki A, Shoda K, Kawaguchi T, Hirajima S, Nagata H, Kubota T, et al: Circulating long non-coding RNAs in plasma of patients with gastric cancer. Anticancer Res. 33:3185–3193. 2013.PubMed/NCBI

10 

Ergun S and Oztuzcu S: Oncocers: ceRNA-mediated cross-talk by sponging miRNAs in oncogenic pathways. Tumor Biol. 36:3129–3136. 2015. View Article : Google Scholar

11 

Cesana M, Cacchiarelli D, Legnini I, Santini T, Sthandier O, Chinappi M, Tramontano A and Bozzoni I: A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA. Cell. 147:358–369. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Chen L, Wang W, Cao L, Li Z and Wang X: Long non-coding RNA CCAT1 acts as a competing endogenous RNA to regulate cell growth and differentiation in acute myeloid leukemia. Mol Cells. 39:330–336. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Lü MH, Tang B, Zeng S, Hu CJ, Xie R, Wu YY, Wang SM, He FT and Yang SM: Long noncoding RNA BC032469, a novel competing endogenous RNA, upregulates hTERT expression by sponging miR-1207-5p and promotes proliferation in gastric cancer. Oncogene. 35:3524–3534. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Chen DL, Ju HQ, Lu YX, Chen LZ, Zeng ZL, Zhang DS, Luo HY, Wang F, Qiu MZ, Wang DS, et al: Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression. J Exp Clin Cancer Res. 35:1422016. View Article : Google Scholar : PubMed/NCBI

15 

Song B, Guan Z, Liu F, Sun D, Wang K and Qu H: Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in gastric cancer cells. Biochem Biophys Res Commun. 464:807–813. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Yan J, Dang Y, Liu S, Zhang Y and Zhang G: LncRNA HOTAIR promotes cisplatin resistance in gastric cancer by targeting miR-126 to activate the PI3K/AKT/MRP1 genes. Tumour Biol. 2016.(Epub ahead of print). View Article : Google Scholar

17 

Yan J: LncRNA HOTAIR acts as a competing endogenous RNA to promote cisplatin resistance in gastric cancer via miR-126/PI3K/AKT/MRP1 pathway. J Gastroenterol Hepatol. 31:53. 2016.PubMed/NCBI

18 

Peng W, Si S, Zhang Q, Li C, Zhao F, Wang F, Yu J and Ma R: Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression. J Exp Clin Cancer Res. 34:792015. View Article : Google Scholar : PubMed/NCBI

19 

Sangodkar J, Shi J, DiFeo A, Schwartz R, Bromberg R, Choudhri A, McClinch K, Hatami R, Scheer E, Kremer-Tal S, et al: Functional role of the KLF6 tumour suppressor gene in gastric cancer. Eur J Cancer. 45:666–676. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Zhang Q, Tan XP, Yuan YS, Hu CM, He CH, Wang WZ, Li JC, Zhao Q and Liu NZ: Decreased expression of KLF6 and its significance in gastric carcinoma. Med Oncol. 27:1295–1302. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Cho YG, Kim CJ, Park CH, Yang YM, Kim SY, Nam SW, Lee SH, Yoo NJ, Lee JY and Park WS: Genetic alterations of the KLF6 gene in gastric cancer. Oncogene. 24:4588–4590. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Zhang X, Nie Y, Du Y, Cao J, Shen B and Li Y: MicroRNA-181a promotes gastric cancer by negatively regulating tumor suppressor KLF6. Tumor Biol. 33:1589–1597. 2012. View Article : Google Scholar

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Zhao L, Han T, Li Y, Sun J, Zhang S, Liu Y, Shan B, Zheng D and Shi J: The lncRNA SNHG5/miR-32 axis regulates gastric cancer cell proliferation and migration by targeting KLF4. FASEB J. 31:893–903. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Zhao H, Peng R, Liu Q, Liu D, Du P, Yuan J, Peng G and Liao Y: The lncRNA H19 interacts with miR-140 to modulate glioma growth by targeting iASPP. Arch Biochem Biophys. 610:1–7. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Wang SH, Wu XC, Zhang MD, Weng MZ, Zhou D and Quan ZW: Long noncoding RNA H19 contributes to gallbladder cancer cell proliferation by modulated miR-194-5p targeting AKT2. Tumor Biol. 37:9721–9730. 2016. View Article : Google Scholar

27 

Wei Y, Sun Q, Zhao L, Wu J, Chen X, Wang Y, Zang W and Zhao G: LncRNA UCA1-miR-507-FOXM1 axis is involved in cell proliferation, invasion and G0/G1 cell cycle arrest in melanoma. Med Oncol. 33:882016. View Article : Google Scholar : PubMed/NCBI

28 

Wang KC, Yang YW, Liu B, Sanyal A, Corces-Zimmerman R, Chen Y, Lajoie BR, Protacio A, Flynn RA, Gupta RA, et al: A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature. 472:120–124. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Guil S and Esteller M: Cis-acting noncoding RNAs: Friends and foes. Nature Struct Mol Biol. 19:1068–1075. 2012. View Article : Google Scholar

30 

Lei Z, Ma X, Li H, Zhang Y, Gao Y, Fan Y, Li X, Chen L, Xie Y, Chen J, et al: Up-regulation of miR-181a in clear cell renal cell carcinoma is associated with lower KLF6 expression, enhanced cell proliferation, accelerated cell cycle transition, and diminished apoptosis. Urol Oncol. 36:93.e23–93.e37. 2018. View Article : Google Scholar

31 

Xu W, He L, Li Y, Tan Y, Zhang F and Xu H: Silencing of lncRNA ZFAS1 inhibits malignancies by blocking Wnt/β-catenin signaling in gastric cancer cells. Biosci Biotechnol Biochem. 82:456–465. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi S, Li D, Li Y, Feng Z, Du Y and Nie Y: LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer. Exp Ther Med 19: 569-578, 2020.
APA
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., & Nie, Y. (2020). LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer. Experimental and Therapeutic Medicine, 19, 569-578. https://doi.org/10.3892/etm.2019.8226
MLA
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., Nie, Y."LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer". Experimental and Therapeutic Medicine 19.1 (2020): 569-578.
Chicago
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., Nie, Y."LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer". Experimental and Therapeutic Medicine 19, no. 1 (2020): 569-578. https://doi.org/10.3892/etm.2019.8226
Copy and paste a formatted citation
x
Spandidos Publications style
Shi S, Li D, Li Y, Feng Z, Du Y and Nie Y: LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer. Exp Ther Med 19: 569-578, 2020.
APA
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., & Nie, Y. (2020). LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer. Experimental and Therapeutic Medicine, 19, 569-578. https://doi.org/10.3892/etm.2019.8226
MLA
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., Nie, Y."LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer". Experimental and Therapeutic Medicine 19.1 (2020): 569-578.
Chicago
Shi, S., Li, D., Li, Y., Feng, Z., Du, Y., Nie, Y."LncRNA CR749391 acts as a tumor suppressor to upregulate KLF6 expression via interacting with miR‑181a in gastric cancer". Experimental and Therapeutic Medicine 19, no. 1 (2020): 569-578. https://doi.org/10.3892/etm.2019.8226
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