Expression and short‑term prognostic value of miR‑126 and miR‑182 in patients with acute stroke

Qi,Ruigang; Liu,Haihua; Liu,Chenglong; Xu,Yingying; Liu,Chunfeng

doi:10.3892/etm.2019.8227

Expression and short‑term prognostic value of miR‑126 and miR‑182 in patients with acute stroke

Authors:
- Ruigang Qi
- Haihua Liu
- Chenglong Liu
- Yingying Xu
- Chunfeng Liu
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Affiliations: Department of Neurology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Neurology, Gaoyou Hospital Affiliated Soochow University, Gaoyou, Jiangsu 225600, P.R. China, Department of Anaesthesiology, Gaoyou Hospital Affiliated Soochow University, Gaoyou, Jiangsu 225600, P.R. China
Published online on: November 21, 2019 https://doi.org/10.3892/etm.2019.8227
Pages: 527-534
Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression and short‑term prognostic value of miR‑126 and miR‑182 in patients with acute stroke were investigated. In total, 153 patients with acute stroke admitted to the Second Affiliated Hospital of Soochow University from February 2016 to February 2018 were enrolled into the observation group as group A [88 patients with acute cerebral infarction (AIS)] or group B [65 patients with cerebral hemorrhage (ICH)]. Furthermore, 69 healthy people receiving physical examinations in the hospital were enrolled into the control group. The relative expression of miR‑126 and miR‑182 in all subjects were measured and their correlation with the National Institute of Health stroke scale (NIHSS) and activities of daily living (ADL) scores was analyzed. After 3 months of follow‑up, the correlation of miR‑126 and miR‑182 with the Modified Rankin Scale (MRS) score of patients was investigated. The receiver operating characteristic (ROC) curve was employed to explore the value of miR‑126 and miR‑182, alone or in combination, in predicting the prognosis of acute stroke patients. Subjects in the control group had markedly higher miR‑126 expression and lower miR‑182 expression than those in group A and group B in the observation group (P<0.05). Pearson's correlation analysis suggested a notable correlation of miR‑126 and miR‑182 with NIHSS and ADL scores. Patients with a mild condition or good prognosis had higher miR‑126 expression and lower miR‑182 expression than patients with a severe condition or poor prognosis (P<0.05). Both miR‑126 and miR‑182 predicted the prognosis of acute stroke, and the combination of miR‑126 and miR‑182 presented better accuracy. The expression levels of miR‑126 and miR‑182 are associated with the neurological function, self‑care ability, and prognosis in patients with acute stroke is highly valuable for predicting the prognosis of patients.

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1	Slivka AP, Notestine MA, Li J and Christoforidis GA: Clinical predictors of cerebrovascular occlusion for patients presenting with acute stroke. J Stroke Cerebrovasc Dis. 15:30–33. 2006. View Article : Google Scholar : PubMed/NCBI
2	Appiah KO, Minhas JS and Robinson TG: Managing high blood pressure during acute ischemic stroke and intracerebral hemorrhage. Curr Opin Neurol. 31:8–13. 2018. View Article : Google Scholar : PubMed/NCBI
3	Lawrence ES, Coshall C, Dundas R, Stewart J, Rudd AG, Howard R and Wolfe CD: Estimates of the prevalence of acute stroke impairments and disability in a multiethnic population. Stroke. 32:1279–1284. 2001. View Article : Google Scholar : PubMed/NCBI
4	Bertog SC, Grunwald IQ, Kühn AL, Vaskelyte L, Hofmann I, Gafoor S, Reinartz M, Matic P and Sievert H: Acute stroke intervention. Interventional Cardiology: Principles and Practice. Second. Dangas GD, Di Mario C, Kipshidze NN, Barlis P, Addo T and Serruys PW: Wiley Online Library; pp. 641–652. 2017
5	Sicras-Mainar A, Planas-Comes A, Frias-Garrido X, Navarro-Artieda R, de Salas-Cansado M and Rejas-Gutiérrez J: Statins after recent stroke reduces recurrence and improves survival in an aging Mediterranean population without known coronary heart disease. J Clin Pharm Ther. 37:441–447. 2012. View Article : Google Scholar : PubMed/NCBI
6	Cao Q, Liu F, Ji K, Liu N, He Y, Zhang W and Wang L: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression. J Exp Clin Cancer Res. 36:292017. View Article : Google Scholar : PubMed/NCBI
7	Chen Y, Song Y, Huang J, Qu M, Zhang Y, Geng J, Zhang Z, Liu J and Yang GY: Increased circulating exosomal miRNA-223 is associated with acute ischemic stroke. Front Neurol. 8:572017. View Article : Google Scholar : PubMed/NCBI
8	Ji B, Wang J, Ma X, Yi YB and Chen ZY: Exosome and miRNA in stroke. Cellular and Molecular Approaches to Regeneration and Repair. Springer; New York, NY: pp. 325–361. 2018
9	Florijn BW, Bijkerk R, van der Veer EP and van Zonneveld AJ: Gender and cardiovascular disease: Are sex-biased microRNA networks a driving force behind heart failure with preserved ejection fraction in women? Cardiovasc Res. 114:210–225. 2018. View Article : Google Scholar : PubMed/NCBI
10	Cheng HL, Fu CY, Kuo WC, Chen YW, Chen YS, Lee YM, Li KH, Chen C, Ma HP, Huang PC, et al: Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system. Lab Chip. 18:2917–2925. 2018. View Article : Google Scholar : PubMed/NCBI
11	Giridharan VV, Quevedo J, Krishnamurthy P and Thandavarayan RA: Editorial commentary: miRNA a tiny genetic tool: Key to the puzzle of cardiovascular disease. Trends Cardiovasc Med. 26:420–422. 2016. View Article : Google Scholar : PubMed/NCBI
12	Maitrias P, Metzinger-Le Meuth V, Nader J, Reix T, Caus T and Metzinger L: The involvement of miRNA in carotid-related stroke. Arterioscler Thromb Vasc Biol. 37:1608–1617. 2017. View Article : Google Scholar : PubMed/NCBI
13	Lee ST, Chu K, Jung KH, Yoon HJ, Jeon D, Kang KM, Park KH, Bae EK, Kim M, Lee SK, et al: MicroRNAs induced during ischemic preconditioning. Stroke. 41:1646–1651. 2010. View Article : Google Scholar : PubMed/NCBI
14	Dunning K: National Institutes of Health Stroke Scale. Encyclopedia of Clinical Neuropsychology. Kreutzer JS, DeLuca J and Caplan B: Springer; New York, NY: pp. 1714–1715. 2011, View Article : Google Scholar
15	Bramlett HM and Dietrich WD: Pathophysiology of cerebral ischemia and brain trauma: Similarities and differences. J Cereb Blood Flow Metab. 24:133–150. 2004. View Article : Google Scholar : PubMed/NCBI
16	Suda S, Katsura K, Kanamaru T, Saito M and Katayama Y: Valproic acid attenuates ischemia-reperfusion injury in the rat brain through inhibition of oxidative stress and inflammation. Eur J Pharmacol. 707:26–31. 2013. View Article : Google Scholar : PubMed/NCBI
17	Liebeskind DS, Sanossian N, Yong WH, Starkman S, Tsang MP, Moya AL, Zheng DD, Abolian AM, Kim D, Ali LK, et al: CT and MRI early vessel signs reflect clot composition in acute stroke. Stroke. 42:1237–1243. 2011. View Article : Google Scholar : PubMed/NCBI
18	Chen X, Yan CC, Zhang X, You ZH, Deng L, Liu Y, Zhang Y and Dai Q: WBSMDA: Within and between score for miRNA-disease association prediction. Sci Rep. 6:211062016. View Article : Google Scholar : PubMed/NCBI
19	Kim JM, Jung KH, Chu K, Lee ST, Ban J, Moon J, Kim M, Lee SK and Roh JK: Atherosclerosis-related circulating MicroRNAs as a predictor of stroke recurrence. Transl Stroke Res. 6:191–197. 2015. View Article : Google Scholar : PubMed/NCBI
20	Cui H and Yang L: Analysis of microRNA expression detected by microarray of the cerebral cortex after hypoxic-ischemic brain injury. J Craniofac Surg. 24:2147–2152. 2013. View Article : Google Scholar : PubMed/NCBI
21	Leung LY, Bartz TM, Rice K, Floyd J, Psaty B, Gutierrez J, Longstreth WT Jr and Mukamal KJ: Blood pressure and heart rate measures associated with increased risk of covert brain infarction and worsening leukoaraiosis in older adults. Arterioscler Thromb Vasc Biol. 37:1579–1586. 2017. View Article : Google Scholar : PubMed/NCBI
22	Sha RJ, Zhang Z, Yu TH, Yuan HL and Icu DO: Etiological factor, risk factor and prognosis in young patients with cerebral infarction and cerebral hemorrhage: comparative analysis among 293 cases. Chin J Clin Rehabil. 26:794–798. 2004.(In Chinese).
23	Wang S, Aurora AB, Johnson BA, Qi X, McAnally J, Hill JA, Richardson JA, Bassel-Duby R and Olson EN: The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis. Dev Cell. 15:261–271. 2008. View Article : Google Scholar : PubMed/NCBI
24	Fish JE, Santoro MM, Morton SU, Yu S, Yeh RF, Wythe JD, Ivey KN, Bruneau BG, Stainier DY and Srivastava D: miR-126 regulates angiogenic signaling and vascular integrity. Dev Cell. 15:272–284. 2008. View Article : Google Scholar : PubMed/NCBI
25	Radom-Aizik S, Zaldivar FP Jr, Haddad F and Cooper DM: Impact of brief exercise on circulating monocyte gene and microRNA expression: Implications for atherosclerotic vascular disease. Brain Behav Immun. 39:121–129. 2014. View Article : Google Scholar : PubMed/NCBI
26	Wang M, Wang W, Wang J and Zhang J: MiR-182 promotes glucose metabolism by upregulating hypoxia-inducible factor 1α in NSCLC cells. Biochem Biophys Res Commun. 504:400–405. 2018. View Article : Google Scholar : PubMed/NCBI
27	Cheng HP, Gong D, Zhao ZW, He PP, Yu XH, Ye Q, Huang C, Zhang X, Chen LY, Xie W, et al: MicroRNA-182 promotes lipoprotein lipase expression and atherogenesis by targeting histone deacetylase 9 in apolipoprotein E-knockout mice. Circ J. 82:28–38. 2017. View Article : Google Scholar : PubMed/NCBI
28	Schmidt MHH, Bicker F, Nikolic I, Meister J, Babuke T, Picuric S, Müller-Esterl W, Plate KH and Dikic I: Epidermal growth factor-like domain 7 (EGFL7) modulates Notch signalling and affects neural stem cell renewal. Nat Cell Biol. 11:873–880. 2009. View Article : Google Scholar : PubMed/NCBI
29	Lee YJ, Bernstock JD, Klimanis D and Hallenbeck JM: Akt protein kinase, miR-200/miR-182 expression and epithelialmesenchymal transition proteins in hibernating ground squirrels. Front Mol Neurosci. 11:222018. View Article : Google Scholar : PubMed/NCBI