miRNA‑429 suppresses osteogenic differentiation of human adipose‑derived mesenchymal stem cells under oxidative stress via targeting SCD‑1 Retraction in /10.3892/etm.2022.11311

Lan,Changgong; Long,Lizhen; Xie,Kegong; Liu,Jia; Zhou,Landao; Pan,Shengcai; Liang,Junqing; Tu,Zhenyang; Gao,Ziran; Tang,Yujin

doi:10.3892/etm.2019.8246

miRNA‑429 suppresses osteogenic differentiation of human adipose‑derived mesenchymal stem cells under oxidative stress via targeting SCD‑1

Retraction in: /10.3892/etm.2022.11311

Authors:
- Changgong Lan
- Lizhen Long
- Kegong Xie
- Jia Liu
- Landao Zhou
- Shengcai Pan
- Junqing Liang
- Zhenyang Tu
- Ziran Gao
- Yujin Tang
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Affiliations: The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, Guangxi 533000, P.R. China
Published online on: November 26, 2019 https://doi.org/10.3892/etm.2019.8246
Pages: 696-702
Copyright: © Lan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Role of microRNA‑429 (miRNA‑429) in osteogenic differentiation of hADMSCs was elucidated to explore the potential mechanism. Serum level of miRNA‑429 in osteoporosis patients and controls was determined by quantitative real‑time polymerase chain reaction (qRT‑PCR). After H2O2 induction in hADMSCs, cell viability and reactive oxygen species (ROS) level were determined by cell‑counting kit (CCK‑8) assay and flow cytometry, respectively. Alkaline phosphatase (ALP) activity in H2O2‑induced hADMSCs was also detected. The binding condition between miRNA‑429 and SCD‑1 was verified by dual‑luciferase reporter gene assay. Relative levels of osteogenesis‑related genes influenced by SCD‑1 and miRNA‑429 were detected by qRT‑PCR. Furthermore, regulatory effects of SCD‑1 and miRNA‑429 on ALP activity and calcification ability of hADMSCs were evaluated. miRNA‑429 was significantly upregulated in serum of osteoporosis patients. During the process of osteogenesis differentiation, H2O2 induction gradually upregulated miRNA‑429 in hADMSCs. Overexpression of miRNA‑429 markedly reduced ALP activity. Subsequent dual‑luciferase reporter gene assay verified that miRNA‑429 could bind to SCD‑1 and negatively regulated its protein level in hADMSCs. SCD‑1 was obviously downregulated in the osteogenesis differentiation of hADMSCs under oxidative stress. Moreover, silencing of SCD‑1 suppressed expression of osteogenesis‑related gene, ALP activity and calcification ability. Notably, SCD‑1 knockdown partially reversed the regulatory effect of miRNA‑429 on the osteogenic differentiation of hADMSCs. miRNA‑429 suppresses the osteogenic differentiation of hADMSCs under oxidative stress via downregulating SCD‑1.

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