Open Access

Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis

  • Authors:
    • Xiaoxuan Liu
    • Zhiguo Wang
    • Wenbin Song
    • Wendong Sun
    • Rundan Hong
    • Anita Pothukuchi
    • Quanchen Xu
  • View Affiliations

  • Published online on: November 29, 2019     https://doi.org/10.3892/etm.2019.8256
  • Pages: 672-682
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gingiva‑derived mesenchymal stem cells (GMSCs) have been the focus of extensive research due to their numerous distinct properties, including their homing to injury sites and their contribution to tissue regeneration. However, the role of transplanted GMSCs in the regulation of lipid metabolism and inflammation in hyperlipidemic mice with periodontitis has not been demonstrated. In the present study, apolipoprotein E‑deficient (ApoE‑/‑) mice were used to establish a hyperlipidemia model with periodontitis and divided into two groups: Group B and Group C (n=20 per group), and wild‑type C57BL/6J mice without any treatment were assigned to Group A (n=20). Animals in Group C were then injected with human GMSCs through the tail vein and animals in Group B were injected with α‑MEM as control. Animals were sacrificed at indicated time points. Serum was collected to determine the lipids and inflammatory cytokines. Liver samples were collected to estimate lipid‑associated gene expression. Morphometric and histological analyses were performed to maxillaries. The results demonstrated that the delivery of GMSCs led to a significant decrease in triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL), interleukin (IL)‑6, tumor necrosis factor (TNF)‑α, alveolar bone loss (ABL), and sterol regulatory element binding protein‑1c (SREBP‑1c) mRNA, and a significant increase in high density lipoprotein cholesterol (HDL), IL‑10 and peroxisome proliferator‑activated receptor α (PPARα) mRNA in Group C compared to Group B. Histological examination showed increased formation of new bone and higher alveolar bone height in Group C. Systematically transplanted GFP‑positive cells were detected through both fluorescence microscope observation and immunohistochemical staining in the periodontal tissues. Overall, systematically transplanted GMSCs attenuated the hyperlipidemia and inflammatory responses in hyperlipidemic mice with periodontitis, and improved periodontal tissue regeneration.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu X, Wang Z, Song W, Sun W, Hong R, Pothukuchi A and Xu Q: Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis. Exp Ther Med 19: 672-682, 2020
APA
Liu, X., Wang, Z., Song, W., Sun, W., Hong, R., Pothukuchi, A., & Xu, Q. (2020). Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis. Experimental and Therapeutic Medicine, 19, 672-682. https://doi.org/10.3892/etm.2019.8256
MLA
Liu, X., Wang, Z., Song, W., Sun, W., Hong, R., Pothukuchi, A., Xu, Q."Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis". Experimental and Therapeutic Medicine 19.1 (2020): 672-682.
Chicago
Liu, X., Wang, Z., Song, W., Sun, W., Hong, R., Pothukuchi, A., Xu, Q."Systematically transplanted human gingiva‑derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis". Experimental and Therapeutic Medicine 19, no. 1 (2020): 672-682. https://doi.org/10.3892/etm.2019.8256