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Article

Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells

  • Authors:
    • Xinhao Wang
    • Hongpeng Zhang
    • Yangyang Ge
    • Jie Liu
    • Dan Rong
    • Long Cao
    • Yuan He
    • Guoyi Sun
    • Senhao Jia
    • Wei Guo
  • View Affiliations / Copyright

    Affiliations: Department of Vascular and Endovascular Surgery, First Medical Center of Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
  • Pages: 748-754
    |
    Published online on: November 29, 2019
       https://doi.org/10.3892/etm.2019.8259
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Abstract

Aortic dissection (AD) is one of the most lethal cardiovascular diseases. Endothelial cell (EC) dysfunction serves an important role in AD progression. Angiotensin II (Ang II) is a key effector in cardiovascular disease development that acts through binding to angiotensin type 1 receptor (AT1R). Yes‑associated protein (YAP) is well‑known as a key mediator of cell proliferation and apoptosis. To determine whether AT1R and YAP influence EC proliferation or injury, human aortic endothelial cells were cultured under different culture conditions. Using CCK‑8 assay, ELISA, western blotting, immunocytochemistry and siRNA transfection, the present study found that Ang II activity reduced EC proliferation, upregulate YAP phosphorylation and result in EC injury that was associated with elevated levels of multiple proinflammatory chemokines. The inhibition of AT1R function, pharmaceutically or via transfection with an AT1R small interfering RNA, alleviated the effects induced by Ang II. Furthermore, AT1R induced YAP phosphorylation via binding to Ang II, and further promoted the inflammation of ECs, along with inhibiting their proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Zhang H, Ge Y, Liu J, Rong D, Cao L, He Y, Sun G, Jia S, Guo W, Guo W, et al: Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells. Exp Ther Med 19: 748-754, 2020.
APA
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L. ... Guo, W. (2020). Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells. Experimental and Therapeutic Medicine, 19, 748-754. https://doi.org/10.3892/etm.2019.8259
MLA
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L., He, Y., Sun, G., Jia, S., Guo, W."Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells". Experimental and Therapeutic Medicine 19.1 (2020): 748-754.
Chicago
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L., He, Y., Sun, G., Jia, S., Guo, W."Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells". Experimental and Therapeutic Medicine 19, no. 1 (2020): 748-754. https://doi.org/10.3892/etm.2019.8259
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Zhang H, Ge Y, Liu J, Rong D, Cao L, He Y, Sun G, Jia S, Guo W, Guo W, et al: Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells. Exp Ther Med 19: 748-754, 2020.
APA
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L. ... Guo, W. (2020). Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells. Experimental and Therapeutic Medicine, 19, 748-754. https://doi.org/10.3892/etm.2019.8259
MLA
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L., He, Y., Sun, G., Jia, S., Guo, W."Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells". Experimental and Therapeutic Medicine 19.1 (2020): 748-754.
Chicago
Wang, X., Zhang, H., Ge, Y., Liu, J., Rong, D., Cao, L., He, Y., Sun, G., Jia, S., Guo, W."Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells". Experimental and Therapeutic Medicine 19, no. 1 (2020): 748-754. https://doi.org/10.3892/etm.2019.8259
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