The preventive effect of Apocynum venetum polyphenols on D‑galactose‑induced oxidative stress in mice

Guo,Huan; Kuang,Zhiping; Zhang,Jing; Zhao,Xin; Pu,Ping; Yan,Junfeng

doi:10.3892/etm.2019.8261

The preventive effect of Apocynum venetum polyphenols on D‑galactose‑induced oxidative stress in mice

Authors:
- Huan Guo
- Zhiping Kuang
- Jing Zhang
- Xin Zhao
- Ping Pu
- Junfeng Yan
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Affiliations: The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, P.R. China, Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, P.R. China, Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China, Department of Internal Medicine‑Neurology, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, P.R. China
Published online on: November 29, 2019 https://doi.org/10.3892/etm.2019.8261
Pages: 557-568
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Apocynum venetum is a traditional medicine that is rich in polyphenols. Apocynum venetum polyphenol extract (AVP) contains the active substances neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid. In the present study, the preventive effect of AVP against D‑galactose‑induced oxidative stress was studied in a mouse model. The sera, skin, livers and spleens of mice were examined using hematoxylin and eosin staining, reverse transcription‑quantitative PCR and western blot analysis. The biochemical results showed that AVP improved the thymus, brain, heart, liver, spleen and kidney indices in a mouse model of oxidative stress. AVP was also able to reverse the reduction in levels of superoxide dismutase (SOD), glutathione peroxidase and glutathione, and increased the levels of nitric oxide and malondialdehyde identified in the serum, liver, spleen and brain of mice exposed to oxidative stress. Pathological observations confirmed that AVP could inhibit oxidative damage to the skin, liver and spleen of mice caused by D‑galactose. Further molecular biological experiments also demonstrated that AVP increased the expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase, Cu/Zn‑SOD, Mn‑SOD, catalase, heme oxygenase‑1, nuclear factor‑erythroid 2‑related factor 2, γ‑glutamylcysteine synthetase and NAD(P)H quinone dehydrogenase 1 and reduced the expression of inducible nitric oxide synthase in the liver and spleen of treated mice compared to controls. Notably, the preventive effect of AVP against D‑galactose‑induced oxidative damage in mice was better than that of the confirmed antioxidant vitamin C. In conclusion, AVP exhibited an antioxidant effect and the AVP‑rich Apocynum venetum may be considered a plant resource with potential antioxidative benefits.

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