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Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α

  • Authors:
    • Kai Zhu
    • Futing Zhao
    • Yanhua Yang
    • Weidong Mu
  • View Affiliations / Copyright

    Affiliations: Department of Traumatic Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Orthopedics, Qingyun County People's Hospital, Dezhou, Shandong 253700, P.R. China, Department of Pathology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 579-584
    |
    Published online on: December 2, 2019
       https://doi.org/10.3892/etm.2019.8267
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Abstract

Effects of simvastatin‑loaded PLGA sustained release microspheres on the treatment of rats with intervertebral disk degeneration (IVDD) and on 6‑keto‑prostaglandin F1α (6‑K‑PGF1α) and hypoxia inducible factor‑1α (HIF‑1α) were investigated. Eighty female rats were selected and randomized into a model group (modeled for IVDD), a treatment group (modeled and treated with simvastatin‑loaded PLGA sustained release microspheres), a sham operation group (only operated without excision), and a control group (not treated) (n=20 each). After modeling, 6‑K‑PGF1α and HIF‑1α in the peripheral blood of the rats were, respectively, detected before simvastatin injection (T0), at 2 weeks (T1) and 4 weeks (T2) after simvastatin injection. The bone mineral density (BMD) of L5 and L6 was detected by X‑ray. The trabecular thickness, number, and separation of the vertebral body were detected. Changes in the sagittal T2‑weighted signal of intervertebral disc nucleus pulposus were detected by MRI. There were no differences between the control and sham operation groups in the indices (P>0.050). Compared with those in the model group during the treatment, BMD, 6‑K‑PGF1α, HIF‑1α, and trabecular number in the treatment group significantly increased (P<0.050), while the trabecular separation significantly decreased (P<0.050). The sagittal T2‑weighted MRI signal in the model group was the lowest between the four groups (P<0.050). Simvastatin‑loaded PLGA sustained release microspheres can improve the BMD of the vertebral body and increase the contents of 6‑K‑PGF1α and HIF‑1α in the treatment of rats with IVDD, so they are important for the clinical treatment of the disease.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu K, Zhao F, Yang Y and Mu W: Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α. Exp Ther Med 19: 579-584, 2020.
APA
Zhu, K., Zhao, F., Yang, Y., & Mu, W. (2020). Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α. Experimental and Therapeutic Medicine, 19, 579-584. https://doi.org/10.3892/etm.2019.8267
MLA
Zhu, K., Zhao, F., Yang, Y., Mu, W."Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α". Experimental and Therapeutic Medicine 19.1 (2020): 579-584.
Chicago
Zhu, K., Zhao, F., Yang, Y., Mu, W."Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α". Experimental and Therapeutic Medicine 19, no. 1 (2020): 579-584. https://doi.org/10.3892/etm.2019.8267
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu K, Zhao F, Yang Y and Mu W: Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α. Exp Ther Med 19: 579-584, 2020.
APA
Zhu, K., Zhao, F., Yang, Y., & Mu, W. (2020). Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α. Experimental and Therapeutic Medicine, 19, 579-584. https://doi.org/10.3892/etm.2019.8267
MLA
Zhu, K., Zhao, F., Yang, Y., Mu, W."Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α". Experimental and Therapeutic Medicine 19.1 (2020): 579-584.
Chicago
Zhu, K., Zhao, F., Yang, Y., Mu, W."Effects of simvastatin‑loaded PLGA microspheres on treatment of rats with intervertebral disk degeneration and on 6‑K‑PGF1α and HIF‑1α". Experimental and Therapeutic Medicine 19, no. 1 (2020): 579-584. https://doi.org/10.3892/etm.2019.8267
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