Synthesis of chemical tools to improve water solubility and promote the delivery of salinomycin to cancer cells

Awad,Loay

doi:10.3892/etm.2019.8368

Synthesis of chemical tools to improve water solubility and promote the delivery of salinomycin to cancer cells

Authors:
- Loay Awad
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Affiliations: Laboratory of Molecular and Chemical Biology of Neurodegeneration, Brain Mind Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne, CH‑1015 Lausanne, Switzerland
Published online on: December 23, 2019 https://doi.org/10.3892/etm.2019.8368
Pages: 1835-1843
Copyright: © Awad . This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chemotherapy and radiation are unable to eliminate all cancer cells, particularly apoptosis‑resistant cancer cells, despite their ability to kill cancer cluster cells. Thus, it is important to identify methods that eliminate all cancer cells in order to prevent relapse. Salinomycin has the ability to control and eradicate different types of cancer, including breast cancer; however, its molecular mechanism remains unclear. The main difficulty in testing salinomycin activity and understanding the governing mechanisms is its low solubility in water (17 mg/l), which can hinder convenient delivery of salinomycin to the protein receptor at the cell surface of stem cells. In the present study, salinomycin was conjugated to the trans‑activator of transcription‑protein in order to facilitate its delivery to the cancer cells. Conjugated salinomycin demonstrated improved solubility in both in vitro. Salinomycin was tested in breast cancer cells (MCF7 and JIMT‑1) by the cleavage of the linker through photolysis at l≥365 nm during in vitro analysis, in the present study.

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