LncRNA LINK‑A regulates ROCK1 expression in early‑stage pancreatic adenocarcinoma

Zhang,Meng; Wang,Rongjun; Zhao,Xun; Lu,Liang; Wang,Tongshan

doi:10.3892/etm.2019.8400

LncRNA LINK‑A regulates ROCK1 expression in early‑stage pancreatic adenocarcinoma

Authors:
- Meng Zhang
- Rongjun Wang
- Xun Zhao
- Liang Lu
- Tongshan Wang
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Affiliations: Department of Oncology, Hospital of Hefei Technology College, Hefei, Anhui 238000, P.R. China, School of Mathematics and Statistics, Nanjing University of Information Science and Technology, Nanjing, Jiangsu 210044, P.R. China, Department of Oncology, Chaohu Hospital of Anhui Medical University, Hefei, Anhui 238001, P.R. China, Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: December 31, 2019 https://doi.org/10.3892/etm.2019.8400
Pages: 1933-1939

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Abstract

Long intergenic non‑coding RNA for kinase activation (LINK‑A) has been characterized as an oncogenic long non‑coding (lnc)RNA in triple‑negative breast cancer and ovarian carcinoma, but its involvement in other malignancies remains elusive. In the present study, it was determined that the plasma levels of LINK‑A lncRNA and Rho‑associated protein kinase 1 (ROCK1) were significantly increased in patients with pancreatic adenocarcinoma compared with those in healthy controls. The plasma levels of LINK‑A lncRNA were positively correlated with the plasma levels of ROCK1 in pancreatic adenocarcinoma patients, but not in healthy controls. Silencing of LINK‑A led to inhibition of pancreatic adenocarcinoma cell proliferation, migration and invasion. Simultaneous overexpression of ROCK1 attenuated the inhibitory effect of LINK‑A silencing on cancer cell proliferation, migration and invasion. Overexpression of LINK‑A lncRNA led to upregulation of ROCK1 expression, while overexpression of ROCK1 had no significant effect on LINK‑A lncRNA expression. It may therefore be concluded that LINK‑A lncRNA may have a role in pancreatic adenocarcinoma, at least in part, by promoting ROCK1 expression.

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