Expression of lncRNA MEG3 in asthma with different phenotypes and its relationship with course of disease

Feng,Yan; Yang,Chang; Yan,Wen

doi:10.3892/etm.2020.8414

Expression of lncRNA MEG3 in asthma with different phenotypes and its relationship with course of disease

Authors:
- Yan Feng
- Chang Yang
- Wen Yan
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Affiliations: Department of Pathology, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, P.R. China, Department of Respiratory Medicine, Hubei No.3 People's Hospital of Jianghan University, Wuhan, Hubei 430030, P.R. China
Published online on: January 3, 2020 https://doi.org/10.3892/etm.2020.8414
Pages: 2211-2217
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of this study was to explore the application value of lncRNA MEG3 in lung cancer. From March 2017 to March 2019, 119 asthma patients and 125 healthy people undergoing physical examination in the same period were selected as the research objects. The levels of lncRNA MEG3 in the peripheral blood of the two groups were compared, and the predictive value of MEG3 for asthma as well as the differences in different inflammatory phenotypes were analyzed. The expression of MEG3 was low in asthma patients (P<0.050), the diagnostic sensitivity and specificity for asthma were 79.83 and 66.40%, respectively (P<0.001), it was the lowest in mixed granulocytic asthma (P<0.050) and was negatively correlated with the course of disease (r=-0.666, P<0.001). Logistic regression analysis showed that course of disease, inflammatory phenotype and MEG3 were independent factors affecting recurrence of asthma (P<0.050). MEG3 was low expressed in asthma and had good predictive value for it; in mixed granulocytic asthma, its expression was the lowest and the course of disease was closely related. It might be the key to the diagnosis and treatment of asthma in the future.

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1	Pavord ID, Beasley R, Agusti A, Anderson GP, Bel E, Brusselle G, Cullinan P, Custovic A, Ducharme FM, Fahy JV, et al: After asthma: Redefining airways diseases. Lancet. 391:350–400. 2018. View Article : Google Scholar : PubMed/NCBI
2	Nunes C, Pereira AM and Morais-Almeida M: Asthma costs and social impact. Asthma Res Pract. 3:12017. View Article : Google Scholar : PubMed/NCBI
3	Winer RA, Qin X, Harrington T, Moorman J and Zahran H: Asthma incidence among children and adults: Findings from the Behavioral Risk Factor Surveillance system asthma call-back survey - United States, 2006–2008. J Asthma. 49:16–22. 2012. View Article : Google Scholar : PubMed/NCBI
4	Loftus PA and Wise SK: Epidemiology of asthma. Curr Opin Otolaryngol Head Neck Surg. 24:245–249. 2016. View Article : Google Scholar : PubMed/NCBI
5	D'Amato G, Vitale C, Molino A, Stanziola A, Sanduzzi A, Vatrella A, Mormile M, Lanza M, Calabrese G, Antonicelli L, et al: Asthma-related deaths. Multidiscip Respir Med. 11:372016. View Article : Google Scholar : PubMed/NCBI
6	Ebmeier S, Thayabaran D, Braithwaite I, Bénamara C, Weatherall M and Beasley R: Trends in international asthma mortality: Analysis of data from the WHO Mortality Database from 46 countries (1993–2012). Lancet. 390:935–945. 2017. View Article : Google Scholar : PubMed/NCBI
7	Horak F, Doberer D, Eber E, Horak E, Pohl W, Riedler J, Szépfalusi Z, Wantke F, Zacharasiewicz A and Studnicka M: Diagnosis and management of asthma - Statement on the 2015 GINA Guidelines. Wien Klin Wochenschr. 128:541–554. 2016. View Article : Google Scholar : PubMed/NCBI
8	Ichinose M, Sugiura H, Nagase H, Yamaguchi M, Inoue H, Sagara H, Tamaoki J, Tohda Y and Munakata M; Japanese Society of Allergology, : Japanese guidelines for adult asthma 2017. Allergol Int. 66:163–189. 2017. View Article : Google Scholar : PubMed/NCBI
9	Paralkar VR, Taborda CC, Huang P, Yao Y, Kossenkov AV, Prasad R, Luan J, Davies JO, Hughes JR, Hardison RC, et al: Unlinking an lncRNA from its associated cis element. Mol Cell. 62:104–110. 2016. View Article : Google Scholar : PubMed/NCBI
10	Nie W, Ge HJ, Yang XQ, Sun X, Huang H, Tao X, Chen WS and Li B: LncRNA-UCA1 exerts oncogenic functions in non-small cell lung cancer by targeting miR-193a-3p. Cancer Lett. 371:99–106. 2016. View Article : Google Scholar : PubMed/NCBI
11	Li W, Zhai L, Wang H, Liu C, Zhang J, Chen W and Wei Q: Downregulation of LncRNA GAS5 causes trastuzumab resistance in breast cancer. Oncotarget. 7:27778–27786. 2016.PubMed/NCBI
12	Boon RA, Hofmann P, Michalik KM, Lozano-Vidal N, Berghäuser D, Fischer A, Knau A, Jaé N, Schürmann C and Dimmeler S: Long noncoding RNA Meg3 controls endothelial cell aging and function: Implications for regenerative angiogenesis. J Am Coll Cardiol. 68:2589–2591. 2016. View Article : Google Scholar : PubMed/NCBI
13	Zhang J, Yao T, Wang Y, Yu J, Liu Y and Lin Z: Long noncoding RNA MEG3 is downregulated in cervical cancer and affects cell proliferation and apoptosis by regulating miR-21. Cancer Biol Ther. 17:104–113. 2016. View Article : Google Scholar : PubMed/NCBI
14	Devadoss D, Long C, Langley RJ, Manevski M, Nair M, Campos MA, Borchert G, Rahman I and Chand HS: Long noncoding transcriptome in chronic obstructive pulmonary disease. Am J Respir Cell Mol Biol. Sep 5–2019.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
15	Borish L: The immunology of asthma: Asthma phenotypes and their implications for personalized treatment. Ann Allergy Asthma Immunol. 117:108–114. 2016. View Article : Google Scholar : PubMed/NCBI
16	Yang IV, Lozupone CA and Schwartz DA: The environment, epigenome, and asthma. J Allergy Clin Immunol. 140:14–23. 2017. View Article : Google Scholar : PubMed/NCBI
17	Burbank AJ, Sood AK, Kesic MJ, Peden DB and Hernandez ML: Environmental determinants of allergy and asthma in early life. J Allergy Clin Immunol. 140:1–12. 2017. View Article : Google Scholar : PubMed/NCBI
18	Quinn JJ, Zhang QC, Georgiev P, Ilik IA, Akhtar A and Chang HY: Rapid evolutionary turnover underlies conserved lncRNA-genome interactions. Genes Dev. 30:191–207. 2016. View Article : Google Scholar : PubMed/NCBI
19	Chak WP, Lung RW, Tong JH, Chan SY, Lun SW, Tsao SW, Lo KW and To KF: Downregulation of long non-coding RNA MEG3 in nasopharyngeal carcinoma. Mol Carcinog. 56:1041–1054. 2017. View Article : Google Scholar : PubMed/NCBI
20	Zhou C, Huang C, Wang J, Huang H, Li J, Xie Q, Liu Y, Zhu J, Li Y, Zhang D, et al: LncRNA MEG3 downregulation mediated by DNMT3b contributes to nickel malignant transformation of human bronchial epithelial cells via modulating PHLPP1 transcription and HIF-1α translation. Oncogene. 36:3878–3889. 2017. View Article : Google Scholar : PubMed/NCBI
21	Xu DH, Chi GN, Zhao CH and Li DY: Long noncoding RNA MEG3 inhibits proliferation and migration but induces autophagy by regulation of Sirt7 and PI3K/AKT/mTOR pathway in glioma cells. J Cell Biochem. 120:7516–7526. 2018. View Article : Google Scholar
22	Wang J, Li F, Yang M, Wu J, Zhao J, Gong W, Liu W, Bi W and Dong L: FIZZ1 promotes airway remodeling through the PI3K/Akt signaling pathway in asthma. Exp Ther Med. 7:1265–1270. 2014. View Article : Google Scholar : PubMed/NCBI
23	Zhang W, Nie Y, Chong L, Cai X, Zhang H, Lin B, Liang Y and Li C: PI3K and Notch signal pathways coordinately regulate the activation and proliferation of T lymphocytes in asthma. Life Sci. 92:890–895. 2013. View Article : Google Scholar : PubMed/NCBI
24	Zhang Y, Jing Y, Qiao J, Luan B, Wang X, Wang L and Song Z: Activation of the mTOR signaling pathway is required for asthma onset. Sci Rep. 7:45322017. View Article : Google Scholar : PubMed/NCBI
25	Bos LD, Sterk PJ and Fowler SJ: Breathomics in the setting of asthma and chronic obstructive pulmonary disease. J Allergy Clin Immunol. 138:970–976. 2016. View Article : Google Scholar : PubMed/NCBI
26	Chung S, Lee TJ, Reader BF, Kim JY, Lee YG, Park GY, Karpurapu M, Ballinger MN, Qian F, Rusu L, et al: FoxO1 regulates allergic asthmatic inflammation through regulating polarization of the macrophage inflammatory phenotype. Oncotarget. 7:17532–17546. 2016. View Article : Google Scholar : PubMed/NCBI
27	Taylor SL, Leong LEX, Choo JM, Wesselingh S, Yang IA, Upham JW, Reynolds PN, Hodge S, James AL, Jenkins C, et al: Inflammatory phenotypes in patients with severe asthma are associated with distinct airway microbiology. J Allergy Clin Immunol. 141:94–103.e15. 2018. View Article : Google Scholar : PubMed/NCBI
28	Chen DL, Shen DY, Han CK and Tian Y: LncRNA MEG3 aggravates palmitate-induced insulin resistance by regulating miR-185-5p/Egr2 axis in hepatic cells. Eur Rev Med Pharmacol Sci. 23:5456–5467. 2019.PubMed/NCBI