Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle

Liu,Yi; Ma,Xiaofang; Chen,Xuhong; Chen,Jinsheng; Yuan,Li; Li,Lili; Bai,Yaowu; Liu,Xiaozhi

doi:10.3892/etm.2020.8416

Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle

Authors:
- Yi Liu
- Xiaofang Ma
- Xuhong Chen
- Jinsheng Chen
- Li Yuan
- Lili Li
- Yaowu Bai
- Xiaozhi Liu
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Affiliations: North China University of Science and Technology, Tangshan, Hebei 063210, P.R. China, Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Department of Gynecology and Obstetrics, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Key Laboratory of Cancer Prevention and Therapy, Department of Bone and Soft Tissue Tumors, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
Published online on: January 3, 2020 https://doi.org/10.3892/etm.2020.8416
Pages: 1855-1863
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The modification of proteins by small ubiquitin‑like modifier (SUMO), known as SUMOylation, regulates biological function by changing protein transcription and translation. During the estrous cycle the endometrium undergoes continual change to processes including cell proliferation, secretion and exfoliation and these changes are regulated by the levels of ovarian hormones. Increasing the expression of SUMO family members has previously been shown to promote proliferation and invasion of endometrial cancer cells. However, limited research has been carried out into the expression and function of SUMO in the mammalian endometrium. In the present study, the level and localization of SUMO‑associated proteins throughout the natural estrous cycle in the mouse uterus was determined using immunohistochemical staining and western blot analysis. The association between the spatiotemporal expression of these SUMO modified proteins and SENPs in endometrium and the concentration of ovarian hormones during estrous cycle was revealed. The present study clarified the role of SUMOylation in maintenance of normal estrous cycling and may have important significance in the study of hormone‑dependent endometrial diseases.

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