Th2 cells as an intermediate for the differentiation of naïve T cells into Th9 cells, associated with the Smad3/Smad4 and IRF4 pathway

  • Authors:
    • Mohamed Hamed Abdelaziz
    • Huixuan Wang
    • Jianjun Cheng
    • Huaxi Xu
  • View Affiliations

  • Published online on: January 3, 2020     https://doi.org/10.3892/etm.2020.8420
  • Pages: 1947-1954
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Abstract

Type 9 T‑helper (Th9) cells are associated with atopic and inflammatory diseases. Their increased levels and functions contribute to a number of inflammatory disorders, where they are accompanied by enhanced Th2‑cell activity. However, there is currently no consensus regarding the association between Th9 and Th2 cells. Th9 cells may be induced from naïve T (Th0) cells under specific polarization conditions in vitro, a process driven by the addition of specific cytokines. In the present study, Th0 cells were cultured under Th9‑polarizing conditions to promote differentiation into interleukin (IL)‑4+ IL‑9‑ or IL‑4‑ IL‑9+ T cells after 3 or 5 days in culture, respectively; the mRNA expression levels of IL‑9 and IL‑4 were consistent with the induced cell types. Simultaneously, the levels of interferon‑regulatory factor 4 (IRF‑4) and Smad3/Smad4 were significantly increased following Th9‑cell polarization. It was therefore proposed that Th2 cells may be generated in the early stages of Th9‑cell differentiation, and then ultimately differentiate into Th9 cells via the Smad3/Smad4 and IRF‑4 activation pathway.
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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Abdelaziz, M.H., Wang, H., Cheng, J., & Xu, H. (2020). Th2 cells as an intermediate for the differentiation of naïve T cells into Th9 cells, associated with the Smad3/Smad4 and IRF4 pathway. Experimental and Therapeutic Medicine, 19, 1947-1954. https://doi.org/10.3892/etm.2020.8420
MLA
Abdelaziz, M. H., Wang, H., Cheng, J., Xu, H."Th2 cells as an intermediate for the differentiation of naïve T cells into Th9 cells, associated with the Smad3/Smad4 and IRF4 pathway". Experimental and Therapeutic Medicine 19.3 (2020): 1947-1954.
Chicago
Abdelaziz, M. H., Wang, H., Cheng, J., Xu, H."Th2 cells as an intermediate for the differentiation of naïve T cells into Th9 cells, associated with the Smad3/Smad4 and IRF4 pathway". Experimental and Therapeutic Medicine 19, no. 3 (2020): 1947-1954. https://doi.org/10.3892/etm.2020.8420