Aldehyde dehydrogenase 1, a target of miR‑222, is expressed at elevated levels in cervical cancer
- Changde Liu
- Yan Zhang
- Shanghua Liang
- Yuhua Ying
Affiliations: Clinical Laboratory, The Hui People Hospital of Beijing, Beijing 100054, P.R. China, Clinical Laboratory, Yuquan Hospital of Tsinghua University, Beijing 100049, P.R. China, Department of Pathology, Beijing Dian Medical Testing Laboratory Co., Beijing 102609, P.R. China, Department of Gynaecology, Yuquan Hospital of Tsinghua University, Beijing 100049, P.R. China
- Published online on: January 7, 2020 https://doi.org/10.3892/etm.2020.8425
Copyright: © Liu
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
The aim of the present study was to investigate the expression of microRNA‑222 (miR‑222) and aldehyde dehydrogenase 1 (ALDH1) in tissues and peripheral blood of cervical cancer patients, and to elucidate their underlying mechanisms of action. Tumor tissues and tumor‑adjacent tissues were obtained from 33 cervical cancer patients and peripheral blood was obtained from these patients and 28 healthy subjects. The expression of miR‑222 and ALDH1 mRNA was evaluated by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). To examine the levels of ALDH1 protein in tissues and blood, western blotting and ELISA were used. To confirm a direct interaction between miR‑222 and ALDH1 mRNA, a dual luciferase reporter assay was performed. HeLA cells were transfected with agomiR‑222 and expression of ALDH1 in the cells was measured by RT‑qPCR and western blotting. MTT assay was preform to investigate the proliferation of HeLA cells. Expression of ALDH1 mRNA and protein was elevated in cervical cancer tissues and peripheral blood from patients compared with tumor‑adjacent tissues and healthy controls, while the expression of miR‑222 was reduced. Upregulation of miR‑222 inhibited HeLA cell proliferation possibly due to a reduction in the expression of ALDH1. A dual luciferase reporter assay showed that miR‑222 can bind with the 3'‑untranslated seed region of ALDH1 mRNA to regulate its expression. miR‑222 regulation of ALDH1 expression may play a role in the prevention of cervical cancer.