Open Access

Raised CD40L expression attenuates drug resistance in Adriamycin‑resistant THP‑1 cells

  • Authors:
    • Zhongxin Feng
    • Qi Chen
  • View Affiliations

  • Published online on: January 15, 2020     https://doi.org/10.3892/etm.2020.8452
  • Pages: 2188-2194
  • Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute myeloid leukemia is a common hematological malignancy that often exhibits strong drug resistance when treated using conventional chemotherapy. Although numerous studies have been carried out to develop methods of overcoming drug resistance, the results have generally been unsatisfactory. CD40 ligand (CD40L) has been shown to improve the sensitivity of cancer cells to drug treatment. In the present study, Adriamycin (ADM)‑resistant human monocytic THP‑1 cells (THP‑1/A cells) were developed by incubating THP‑1 cells with increasing concentrations of ADM. Cells were transfected with CD40L vectors to explore the potential involvement of CD40L in regulating multidrug resistance (MDR) in cancer. Cell proliferation and viability were measured using the Cell Counting Kit‑8 assay; cell apoptosis was evaluated by flow cytometry, trypan blue staining and caspase‑3 activity; and the expression of MDR‑associated protein 1 (MRP1) and permeability glycoprotein (P‑gp) was analyzed using western blotting. The results revealed that the protein expression levels of MRP1 and P‑gp were downregulated by raised CD40L expression and that the combination of raised CD40L expression with daunorubicin (DNR), a drug from which ADM is derived, significantly increased the extent of cell apoptosis, indicating that drug resistance was effectively attenuated by CD40L. Collectively, these results suggested that CD40L may contribute towards reducing DNR resistance in THP‑1/A cells.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Feng, Z., & Feng, Z. (2020). Raised CD40L expression attenuates drug resistance in Adriamycin‑resistant THP‑1 cells. Experimental and Therapeutic Medicine, 19, 2188-2194. https://doi.org/10.3892/etm.2020.8452
MLA
Feng, Z., Chen, Q."Raised CD40L expression attenuates drug resistance in Adriamycin‑resistant THP‑1 cells". Experimental and Therapeutic Medicine 19.3 (2020): 2188-2194.
Chicago
Feng, Z., Chen, Q."Raised CD40L expression attenuates drug resistance in Adriamycin‑resistant THP‑1 cells". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2188-2194. https://doi.org/10.3892/etm.2020.8452