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Article Open Access

miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway

Corrigendum in: /10.3892/etm.2020.9412
  • Authors:
    • Shaohua Xiang
    • Jing Li
    • Zhengfu Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Dianjiang County Hospital of Traditional Chinese Medicine, Chongqing 408300, P.R. China, Department of Cardiothoracic Surgery, People's Hospital of Changshou, Chongqing 401220, P.R. China
    Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2067-2074
    |
    Published online on: January 15, 2020
       https://doi.org/10.3892/etm.2020.8455
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Abstract

A critical event in cardiac fibrosis is the transformation of cardiac fibroblasts (CFs) into myofibroblasts. MicroRNAs (miRNAs) have been reported to be critical regulators in the development of cardiac fibrosis. However, the underlying molecular mechanisms of action of miRNA (miR)‑26b in cardiac fibrosis have not yet been extensively studied. In the present study, the expression levels of miR‑26b were downregulated in isoproterenol (ISO)‑treated cardiac tissues and CFs. Moreover, miR‑26b overexpression inhibited the cell viability of ISO‑treated CFs and decreased the protein levels of collagen I and α‑smooth muscle actin (α‑SMA). Furthermore, bioinformatics analysis and dual luciferase reporter assays indicated that Kelch‑like ECH‑associated protein 1 (Keap1) was the target of miR‑26b, and that its expression levels were decreased in miR‑26b‑treated cells. In addition, Keap1 overexpression reversed the inhibitory effects of miR‑26b on ISO‑induced cardiac fibrosis, as demonstrated by cell viability, and the upregulation of collagen I and α‑SMA expression levels. Furthermore, inhibition of Keap1 expression led to the activation of nuclear factor erythroid 2‑related factor 2 (Nrf2), which induced the transcriptional activation of antioxidant/detoxifying proteins in order to protect against cardiac fibrosis. Taken together, the data demonstrated that miR‑26b attenuated ISO‑induced cardiac fibrosis via the Keap‑mediated activation of Nrf2.
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Copy and paste a formatted citation
Spandidos Publications style
Xiang S, Li J and Zhang Z: miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412. Exp Ther Med 19: 2067-2074, 2020.
APA
Xiang, S., Li, J., & Zhang, Z. (2020). miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412. Experimental and Therapeutic Medicine, 19, 2067-2074. https://doi.org/10.3892/etm.2020.8455
MLA
Xiang, S., Li, J., Zhang, Z."miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412". Experimental and Therapeutic Medicine 19.3 (2020): 2067-2074.
Chicago
Xiang, S., Li, J., Zhang, Z."miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2067-2074. https://doi.org/10.3892/etm.2020.8455
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang S, Li J and Zhang Z: miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412. Exp Ther Med 19: 2067-2074, 2020.
APA
Xiang, S., Li, J., & Zhang, Z. (2020). miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412. Experimental and Therapeutic Medicine, 19, 2067-2074. https://doi.org/10.3892/etm.2020.8455
MLA
Xiang, S., Li, J., Zhang, Z."miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412". Experimental and Therapeutic Medicine 19.3 (2020): 2067-2074.
Chicago
Xiang, S., Li, J., Zhang, Z."miR‑26b inhibits isoproterenol‑induced cardiac fibrosis via the Keap1/Nrf2 signaling pathway Corrigendum in /10.3892/etm.2020.9412". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2067-2074. https://doi.org/10.3892/etm.2020.8455
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