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Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model

  • Authors:
    • Ying Guan
    • Lifeng Li
    • Jianqiang Chen
    • Hong Lu
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasonography, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570102, P.R. China, Department of Radiology, Changsha Central Hospital, Changsha, Hunan 410004, P.R. China, Department of Radiology, Haikou People's Hospital, Haikou, Hainan 570208, P.R. China, Department of Radiology, The Seventh People's Hospital of Chongqing, Chongqing 400054, P.R. China
    Copyright: © Guan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2029-2036
    |
    Published online on: January 15, 2020
       https://doi.org/10.3892/etm.2020.8456
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Abstract

Traumatic brain injury (TBI) is one of the leading causes of mortality and permanent disabilities worldwide. Brain edema following TBI remains to be the predominant cause of mortality and disability in patients worldwide. Previous studies have reported that brain edema is closely associated with aquaporin‑4 (AQP4) expression. AQP4 is a water channel protein and mediates water homeostasis in a variety of brain disorders. In the current study, a rat TBI model was established, and the features of brain edema following TBI were assessed using multimodal MRI. The results of the multimodal MRI were useful, reliable and were used to evaluate the extent and the type of brain edema following TBI. Brain edema was also successfully alleviated using an intracerebral injection of AQP4 small interfering (si)RNA. The expression of AQP4 and its role in brain edema were also examined in the present study. The AQP4 siRNA was demonstrated to downregulate AQP4 expression following TBI and reduced brain edema at the early stages of TBI (6 and 12 h). The current study revealed the MRI features of brain edema and the changes in AQP4 expression exhibited following TBI, and the results provide important information that can be used to improve the early diagnosis and treatment of brain edema.
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1 

Stein DM, Feather CB and Napolitano LM: Traumatic brain injury advances. Crit Care Clin. 33:1–13. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Fukuda AM, Adami A, Pop V, Bellone JA, Coats JS, Hartman RE, Ashwal S, Obenaus A and Badaut J: Posttraumatic reduction of edema with aquaporin-4 RNA interference improves acute and chronic functional recovery. J Cereb Blood Flow Metab. 33:1621–1632. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Marmarou A, Fatouros PP, Barzó P, Portella G, Yoshihara M, Tsuji O, Yamamoto T, Laine F, Signoretti S, Ward JD, et al: Contribution of edema and cerebral blood volume to traumatic brain swelling in head-injured patients. J Neurosurg. 93:183–193. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Kunkel A, Filik R, Mackenzie IG and Leuthold H: Task-dependent evaluative processing of moral and emotional content during comprehension: An ERP study. Cogn Affect Behav Neurosci. 18:389–409. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Nicchia GP, Frigeri A, Liuzzi GM and Svelto M: Inhibition of aquaporin-4 expression in astrocytes by RNAi determines alteration in cell morphology, growth, and water transport and induces changes in ischemia-related genes. FASEB J. 17:1508–1510. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Lescot T, Fulla-Oller L, Po C, Chen XR, Puybasset L, Gillet B, Plotkine M, Meric P and Marchand-Leroux C: Temporal and regional changes after focal traumatic brain injury. J Neurotrauma. 27:85–94. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Maegele M, Stuermer EK, Hoeffgen A, Uhlenkueken U, Mautes A, Schaefer N, Lippert-Gruener M, Schaefer U and Hoehn M: Multimodal MR imaging of acute and subacute experimental traumatic brain injury: Time course and correlation with cerebral energy metabolites. Acta Radiol Short Rep. 4:20479816145551422015.PubMed/NCBI

8 

Xu S, Zhuo J, Racz J, Shi D, Roys S, Fiskum G and Gullapalli R: Early microstructural and metabolic changes following controlled cortical impact injury in rat: A magnetic resonance imaging and spectroscopy study. J Neurotrauma. 28:2091–2102. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Tang G and Yang GY: Aquaporin-4: A potential therapeutic target for cerebral edema. Int J Mol Sci. 17(pii): E14132016. View Article : Google Scholar : PubMed/NCBI

10 

Manley GT, Fujimura M, Ma T, Noshita N, Filiz F, Bollen AW, Chan P and Verkman AS: Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke. Nat Med. 6:159–163. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Vajda Z, Promeneur D, Dóczi T, Sulyok E, Frøkiaer J, Ottersen OP and Nielsen S: Increased aquaporin-4 immunoreactivity in rat brain in response to systemic hyponatremia. Biochem Biophys Res Commun. 270:495–503. 2000. View Article : Google Scholar : PubMed/NCBI

12 

McIntosh TK, Vink R, Noble L, Yamakami I, Fernyak S, Soares H and Faden AL: Traumatic brain injury in the rat: Characterization of a lateral fluid-percussion model. Neuroscience. 28:233–244. 1989. View Article : Google Scholar : PubMed/NCBI

13 

Badaut J, Fukuda AM, Jullienne A and Petry KG: Aquaporin and brain diseases. Biochim Biophys Acta. 1840:1554–1565. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wei XE, Zhang YZ, Li YH, Li MH and Li WB: Dynamics of rabbit brain edema in focal lesion and perilesion area after traumatic brain injury: A MRI study. J Neurotrauma. 29:2413–2420. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Tourdias T, Dragonu I, Fushimi Y, Deloire MS, Boiziau C, Brochet B, Moonen C, Petry KG and Dousset V: Aquaporin 4 correlates with apparent diffusion coefficient and hydrocephalus severity in the rat brain: A combined MRI-histological study. Neuroimage. 47:659–666. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Hudak AM, Peng L, Marquez de la Plata C, Thottakara J, Moore C, Harper C, McColl R, Babcock E and Diaz-Arrastia R: Cytotoxic and vasogenic cerebral oedema in traumatic brain injury: Assessment with FLAIR and DWI imaging. Brain Inj. 28:1602–1609. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Gatidis S, Schmidt H, Martirosian P, Nikolaou K and Schwenzer NF: Apparent diffusion coefficient-dependent voxelwise computed diffusion-weighted imaging: An approach for improving SNR and reducing T2 shine-through effects. J Magn Reson Imaging. 43:824–832. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Oshio K, Okuda S and Shinmoto H: Removing ambiguity caused by T2 Shine-through using weighted diffusion subtraction (WDS). Magn Reson Med Sci. 15:146–148. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Hsu CC, Watkins TW, Kwan GN and Haacke EM: Susceptibility-weighted imaging of Glioma: Update on current imaging status and future directions. J Neuroimaging. 26:383–390. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Chen JQ, Zhang CC, Jiang SN, Lu H and Wang W: Effects of aquaporin 4 knockdown on brain edema of the uninjured side after traumatic brain injury in rats. Med Sci Monit. 22:4809–4819. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Tang G, Liu Y, Zhang Z, Lu Y, Wang Y, Huang J, Li Y, Chen X, Gu X, Wang Y, et al: Mesenchymal stem cells maintain blood-brain barrier integrity by inhibiting aquaporin-4 upregulation after cerebral ischemia. Stem Cells. 32:3150–3162. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Vindedal GF, Thoren AE, Jensen V, Klungland A, Zhang Y, Holtzman MJ, Ottersen OP and Nagelhus EA: Removal of aquaporin-4 from glial and ependymal membranes causes brain water accumulation. Mol Cell Neurosci. 77:47–52. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Yang B, Zador Z and Verkman AS: Glial cell aquaporin-4 overexpression in transgenic mice accelerates cytotoxic brain swelling. J Biol Chem. 283:15280–15286. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Higashida T, Kreipke CW, Rafols JA, Peng C, Schafer S, Schafer P, Ding JY, Dornbos D III, Li X, Guthikonda M, et al: The role of hypoxia-inducible factor-1α, aquaporin-4 and matrix metalloproteinase-9 in blood-brain barrier disruption and brain edema after traumatic brain injury. J Neurosurg. 114:92–101. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Lu H, Lei XY, Hu H and He ZP: Relationship between AQP4 expression and structural damage to the blood-brain barrier at early stages of traumatic brain injury in rats. Chin Med J (Engl). 126:4316–4321. 2013.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guan Y, Li L, Chen J and Lu H: Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model. Exp Ther Med 19: 2029-2036, 2020.
APA
Guan, Y., Li, L., Chen, J., & Lu, H. (2020). Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model. Experimental and Therapeutic Medicine, 19, 2029-2036. https://doi.org/10.3892/etm.2020.8456
MLA
Guan, Y., Li, L., Chen, J., Lu, H."Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model". Experimental and Therapeutic Medicine 19.3 (2020): 2029-2036.
Chicago
Guan, Y., Li, L., Chen, J., Lu, H."Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2029-2036. https://doi.org/10.3892/etm.2020.8456
Copy and paste a formatted citation
x
Spandidos Publications style
Guan Y, Li L, Chen J and Lu H: Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model. Exp Ther Med 19: 2029-2036, 2020.
APA
Guan, Y., Li, L., Chen, J., & Lu, H. (2020). Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model. Experimental and Therapeutic Medicine, 19, 2029-2036. https://doi.org/10.3892/etm.2020.8456
MLA
Guan, Y., Li, L., Chen, J., Lu, H."Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model". Experimental and Therapeutic Medicine 19.3 (2020): 2029-2036.
Chicago
Guan, Y., Li, L., Chen, J., Lu, H."Effect of AQP4‑RNAi in treating traumatic brain edema: Multi‑modal MRI and histopathological changes of early stage edema in a rat model". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2029-2036. https://doi.org/10.3892/etm.2020.8456
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