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Effects of tenuifolin on rest/wake behaviour in zebrafish

  • Authors:
    • Zi‑Wen Chen
    • Chao‑Bao Peng
    • Zhong Pei
    • Meng‑Ruo Zhang
    • Tian‑Chan Yun
    • Zhi‑Min Yang
    • Fu‑Ping Xu
  • View Affiliations / Copyright

    Affiliations: Health Construction Administration Center, The Second Clinical Medicine College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Traditional Chinese Medicine, Zhaoqing Hospital of Traditional Chinese Medicine, Zhaoqing, Guangdong 526020, P.R. China, Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Health Construction Administration Center, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2326-2334
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    Published online on: January 28, 2020
       https://doi.org/10.3892/etm.2020.8476
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Abstract

Insomnia is a common sleep disorder with a high prevalence and substantial adverse consequences. There is growing interest in identifying novel therapeutics from herbal medicine. Tenuifolin is a major constituent of the well‑known anti‑insomnia herb Radix Polygala. The present study investigated the neural activity in response to tenuifolin during rest/wake behaviour in zebrafish and identified the potential biological signalling pathways involved. An automatic video tracking system was used to monitor the behavioural response of zebrafish larvae for 24 h after treatment with tenuifolin. In total, six rest/wake parameters were measured and visualized with a behavioural fingerprint. Time series analysis was conducted by averaging the total rest and waking activity in 10 min intervals. A correlation analysis was performed between tenuifolin and well‑known compounds to analyse the underlying biological signalling pathways. Reverse transcription‑quantitative PCR was also performed to detect the effects of tenuifolin on the transcription of interesting genes associated with the signalling pathways that were potentially involved. The present results suggested tenuifolin significantly increased the total rest time during the dark phase, with a slight effect on the waking activity in zebrafish larvae. This behavioural phenotype induced by tenuifolin is similar to that of selective serotonin reuptake inhibitors and gamma‑aminobutyric acid (GABA) agonists. Furthermore, the expression levels of GABA transporter 1 were significantly increased after tenuifolin treatment. No significant difference was determined in other associated genes in untreated control and tenuifolin‑treated larvae. The present results suggested that tenuifolin caused sleep‑promoting activity in zebrafish and that these effects may be mediated by the serotoninergic systems and the GABAergic systems.
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Copy and paste a formatted citation
Spandidos Publications style
Chen ZW, Peng CB, Pei Z, Zhang MR, Yun TC, Yang ZM and Xu FP: Effects of tenuifolin on rest/wake behaviour in zebrafish. Exp Ther Med 19: 2326-2334, 2020.
APA
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., & Xu, F. (2020). Effects of tenuifolin on rest/wake behaviour in zebrafish. Experimental and Therapeutic Medicine, 19, 2326-2334. https://doi.org/10.3892/etm.2020.8476
MLA
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., Xu, F."Effects of tenuifolin on rest/wake behaviour in zebrafish". Experimental and Therapeutic Medicine 19.3 (2020): 2326-2334.
Chicago
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., Xu, F."Effects of tenuifolin on rest/wake behaviour in zebrafish". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2326-2334. https://doi.org/10.3892/etm.2020.8476
Copy and paste a formatted citation
x
Spandidos Publications style
Chen ZW, Peng CB, Pei Z, Zhang MR, Yun TC, Yang ZM and Xu FP: Effects of tenuifolin on rest/wake behaviour in zebrafish. Exp Ther Med 19: 2326-2334, 2020.
APA
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., & Xu, F. (2020). Effects of tenuifolin on rest/wake behaviour in zebrafish. Experimental and Therapeutic Medicine, 19, 2326-2334. https://doi.org/10.3892/etm.2020.8476
MLA
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., Xu, F."Effects of tenuifolin on rest/wake behaviour in zebrafish". Experimental and Therapeutic Medicine 19.3 (2020): 2326-2334.
Chicago
Chen, Z., Peng, C., Pei, Z., Zhang, M., Yun, T., Yang, Z., Xu, F."Effects of tenuifolin on rest/wake behaviour in zebrafish". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2326-2334. https://doi.org/10.3892/etm.2020.8476
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