Open Access

Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis

  • Authors:
    • He‑Ping Li
    • Chun‑Mei Xu
    • Bing‑Yan Wen
    • An‑Qi Li
    • Guang‑Ming Zha
    • Xiang‑Yang Jin
    • Yun‑Ze Zhao
    • Lu‑Ping Feng
    • Ye‑Dong Cao
    • Guo‑Yu Yang
    • Yue‑Ying Wang
    • Kai Zhong
  • View Affiliations

  • Published online on: January 28, 2020     https://doi.org/10.3892/etm.2020.8477
  • Pages: 2149-2154
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Trefoil factor 3 (TFF3) is involved in cell adhesion, motility and apoptosis, regulates mucosal immunity and maintains the functional integrity of intestinal epithelia. The upregulation of TFF3 expression in the weaning rat intestine attracted our interest. The present study hypothesized that TFF3 may serve a role in preventing diarrhea in weaning piglets, which is an important consideration in the pig farming industry. Previous recombinant TFF3 protein expression yields obtained from Escherichia coli were too low and the bioactivity of the protein was poor. Hence, this expression system was unsuitable for industrial applications. The present study explored the production of recombinant sus scrofa TFF3 in a Brevibacillus choshinensis (B. choshinensis) expression system, aiming to enhance the expression level of bioactive protein. To achieve this, the sus scrofa TFF3‑encoding gene fragment was fused into an E. coli‑Brevibacillus shuttle vector pNCMO2. High levels of TFF3 (30 mg/l) were produced and secreted into the B. choshinensis culture medium in soluble form with a molecular mass of 13.6 kDa and high immunoreactivity in western blotting. Thus, Brevibacillus may be used to produce useful mucosal factors for biochemical analyses and mucosal protection, and in industrial applications to produce novel inhibitors of diarrhea.
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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Li, H., Xu, C., Wen, B., Li, A., Zha, G., Jin, X. ... Zhong, K. (2020). Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis. Experimental and Therapeutic Medicine, 19, 2149-2154. https://doi.org/10.3892/etm.2020.8477
MLA
Li, H., Xu, C., Wen, B., Li, A., Zha, G., Jin, X., Zhao, Y., Feng, L., Cao, Y., Yang, G., Wang, Y., Zhong, K."Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis". Experimental and Therapeutic Medicine 19.3 (2020): 2149-2154.
Chicago
Li, H., Xu, C., Wen, B., Li, A., Zha, G., Jin, X., Zhao, Y., Feng, L., Cao, Y., Yang, G., Wang, Y., Zhong, K."Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2149-2154. https://doi.org/10.3892/etm.2020.8477