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Article

MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1

  • Authors:
    • Xiaoxue Shen
    • Zhibin Zhao
    • Bin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Jiangsu Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China
  • Pages: 2725-2732
    |
    Published online on: February 4, 2020
       https://doi.org/10.3892/etm.2020.8485
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Abstract

Colonic dysmotility as a complication of diabetes affects public health; however, the underlying molecular mechanisms have remained elusive. Insulin‑like growth factor‑1 (IGF‑1) was previously demonstrated to prevent apoptosis of colonic smooth muscle cells (SMCs) and alleviate colonic dysmotility in diabetic rats. However, the regulatory mechanisms upstream of IGF‑1 in colonic dysmotility have remained to be determined. The present study reports on microRNA‑155 (miR‑155), initially identified using bioinformatics, as a direct upstream regulator of IGF‑1. In colonic SMCs, miR‑155 negatively regulated IGF‑1 expression at the post‑transcriptional level, as identified through ectopic overexpression and knockdown experiments. A luciferase reporter assay further demonstrated that miR‑155 inhibits IGF‑1 through binding to its 3'‑untranslated region. Furthermore, overexpression of miR‑155 led to increased apoptosis of colonic SMCs and a decrease in the thickness of colonic smooth muscle tissues of diabetic mice, indicating miR‑155 aggravates colonic dysmotility. By contrast, knockdown of miR‑155 induced the opposite effect. Overall, the results of the present study suggest a role of miR‑155 in colonic dysmotility, thereby providing a novel therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Shen X, Zhao Z and Yang B: MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1. Exp Ther Med 19: 2725-2732, 2020.
APA
Shen, X., Zhao, Z., & Yang, B. (2020). MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1. Experimental and Therapeutic Medicine, 19, 2725-2732. https://doi.org/10.3892/etm.2020.8485
MLA
Shen, X., Zhao, Z., Yang, B."MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1". Experimental and Therapeutic Medicine 19.4 (2020): 2725-2732.
Chicago
Shen, X., Zhao, Z., Yang, B."MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2725-2732. https://doi.org/10.3892/etm.2020.8485
Copy and paste a formatted citation
x
Spandidos Publications style
Shen X, Zhao Z and Yang B: MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1. Exp Ther Med 19: 2725-2732, 2020.
APA
Shen, X., Zhao, Z., & Yang, B. (2020). MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1. Experimental and Therapeutic Medicine, 19, 2725-2732. https://doi.org/10.3892/etm.2020.8485
MLA
Shen, X., Zhao, Z., Yang, B."MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1". Experimental and Therapeutic Medicine 19.4 (2020): 2725-2732.
Chicago
Shen, X., Zhao, Z., Yang, B."MicroRNA‑155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF‑1". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2725-2732. https://doi.org/10.3892/etm.2020.8485
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