Decreased basal and stimulated salivary parameters by histopathological lesions and secretory dysfunction of parotid and submandibular glands in rats with type 2 diabetes

Chen,Si‑Yu; Wang,Ying; Zhang,Chun‑Lei; Yang,Ze‑Min

doi:10.3892/etm.2020.8505

Decreased basal and stimulated salivary parameters by histopathological lesions and secretory dysfunction of parotid and submandibular glands in rats with type 2 diabetes

Authors:
- Si‑Yu Chen
- Ying Wang
- Chun‑Lei Zhang
- Ze‑Min Yang
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Affiliations: Department of Biochemistry and Molecular Biology, School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China
Published online on: February 10, 2020 https://doi.org/10.3892/etm.2020.8505
Pages: 2707-2719
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Patients with type 2 diabetes mellitus (T2DM) present with dry mouth, polydipsia and taste impairment due to salivary secretion disorder. However, the underlying functional mechanism of T2DM remains unknown. The present study found that T2DM rats had significantly lower salivary flow rate and salivary alpha amylase activity, and attenuated salivary secretion responses to acid stimulation compared with control rats. Histopathological observation found that T2DM rats had inflammatory cell infiltration with increased expressions of IL‑6 and TNF‑α, oxidative stress, including decreased total superoxide dismutase activity and increased malondialdehyde content, and decreased expressions of β1 adrenergic receptor, cholinergic receptor, aquaporin‑5 and protein kinase A in salivary glands, in particular the parotid gland. These results indicated that parotid gland impairment was more severe compared with submandibular gland impairment. Reduced salivary secretion may be associated with histopathological lesions and decreased regulation in secretory pathways in salivary glands.

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