miR‑200b regulates breast cancer cell proliferation and invasion by targeting radixin

Yuan,Jianfen; Xiao,Chunhong; Lu,Huijun; Yu,Haizhong; Hong,Hong; Guo,Chunyan; Wu,Zhimei

doi:10.3892/etm.2020.8516

miR‑200b regulates breast cancer cell proliferation and invasion by targeting radixin

Authors:
- Jianfen Yuan
- Chunhong Xiao
- Huijun Lu
- Haizhong Yu
- Hong Hong
- Chunyan Guo
- Zhimei Wu
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Affiliations: Department of Clinical Laboratory, Nantong Traditional Chinese Medicine Hospital, Nantong, Jiangsu 226001, P.R. China, Department of Clinical Laboratory, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China
Published online on: February 11, 2020 https://doi.org/10.3892/etm.2020.8516
Pages: 2741-2750

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Abstract

Radixin is an important member of the Ezrin‑Radixin‑Moesin protein family that is involved in cell invasion, metastasis and movement. microRNA (miR)‑200b is a well‑studied microRNA associated with the development of multiple tumors. Previous bioinformatics analysis has demonstrated that miR‑200b has a complementary binding site in the 3'‑untranslated region of radixin mRNA. The present study aimed to investigate the role of miR‑200b in regulating radixin expression, cell proliferation and invasion in breast cancer. Breast cancer tissues at different Tumor‑Node‑Metastasis (TNM) stages were collected; breast tissues from patients with hyperplasia were used as a control. miR‑200b and radixin mRNA expression levels were tested by reverse transcription‑quantitative PCR. Radixin protein expression was detected by western blotting. The highly metastatic MDA‑MB‑231 cells were divided into four groups and transfected with a miR‑negative control (NC), miR‑200b mimic, small interfering (si)RNA‑NC or siRNA targeting radixin. Cell invasion was evaluated by Transwell assay and cell proliferation was assessed by 5‑ethynyl‑2'‑deoxyuridine staining. Compared with the control group, radixin mRNA expression was significantly higher in breast cancer tissues and increased with TNM stage. miR‑200b expression levels exhibited the opposite trend. Radixin mRNA expression in breast cancer cells was notably higher, whereas miR‑200b expression was lower compared with that in normal breast epithelial MCF‑10A cells. The expression of radixin was higher, whereas miR‑200b was lower in MDA‑MB‑231 cells compared with that in MCF‑7 cells. miR‑200b mimic or siRNA‑radixin transfection downregulated the expression of radixin in MDA‑MB‑231 cells and attenuated the invasive and proliferative abilities of these cells. miR‑200b‑knockdown and radixin overexpression were associated with enhanced cell invasion in breast cancer. In conclusion, miR‑200b regulates breast cancer cell proliferation and invasion by targeting radixin expression.

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