Open Access

miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma

  • Authors:
    • Maosong Wu
    • Yongsheng Tang
    • Gang Hu
    • Chunjian Yang
    • Kaichuang Ye
    • Xianluo Liu
  • View Affiliations

  • Published online on: February 25, 2020     https://doi.org/10.3892/etm.2020.8546
  • Pages: 3017-3023
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hemangiomas (HAs) are benign neoplasms of the vasculature. MicroRNA‑4458 (miR‑4458) has been reported to function as a tumor suppressor in multiple malignancies, but its biological function in HAs remains unknown. In the present study, the potential role of miR‑4458 in HA‑derived endothelial cells (HDECs) was investigated. Firstly, reverse‑transcription‑quantitative PCR analysis was used to confirm the expression of miR‑4458 in HDECs following transfection with miR‑4458 mimics or inhibitor. Subsequently, MTT and EdU assays were performed and subsequently determined that miR‑4458 overexpression significantly inhibited proliferation, and knockdown promoted cell proliferation in HDECs. Flow cytometry analysis revealed that miR‑4458 overexpression induced cell cycle arrest, whereas knockdown reversed G0/G1 phase arrest and apoptosis. Furthermore, insulin‑like growth factor 1 receptor (IGF1R) was identified as a target of miR‑4458. IGF1R knockdown enhanced the effects of miR‑4458 on cell proliferation, cell cycle G0/G1 phase arrest and apoptosis in HDECs. Taken together, the results revealed that miR‑4458 targeting of IGF1R may serve as a novel therapeutic strategy for treating patients with HAs.
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April-2020
Volume 19 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu M, Tang Y, Hu G, Yang C, Ye K and Liu X: miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma. Exp Ther Med 19: 3017-3023, 2020
APA
Wu, M., Tang, Y., Hu, G., Yang, C., Ye, K., & Liu, X. (2020). miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma. Experimental and Therapeutic Medicine, 19, 3017-3023. https://doi.org/10.3892/etm.2020.8546
MLA
Wu, M., Tang, Y., Hu, G., Yang, C., Ye, K., Liu, X."miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma". Experimental and Therapeutic Medicine 19.4 (2020): 3017-3023.
Chicago
Wu, M., Tang, Y., Hu, G., Yang, C., Ye, K., Liu, X."miR‑4458 directly targets IGF1R to inhibit cell proliferation and promote apoptosis in hemangioma". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3017-3023. https://doi.org/10.3892/etm.2020.8546