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lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway

  • Authors:
    • Xiaojin Yang
    • Hanchuan Tao
    • Cheng Wang
    • Weijun Chen
    • Fu Hua
    • Haixin Qian
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, First Affiliated Hospital of Soochow University, Soochow, Jiangsu 215006, P.R. China, Department of Neurology, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China, Department of General Surgery, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3097-3103
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    Published online on: February 26, 2020
       https://doi.org/10.3892/etm.2020.8558
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Abstract

Long non‑coding RNA activated by transforming growth factor‑β (ATB) was recently reported to be involved in a wide range of physiological and pathological processes. However, the role of ATB in colorectal cancer (CRC) stemness remains unclear. In the present study, the functional role of ATB in maintaining stemness of CRC was determined using colony formation and sphere formation assays, and xenograft models. Reverse transcription‑quantitative PCR, western blotting and immunohistochemistry were performed to investigate the mechanisms underlying the effects of ATB. Knockdown of ATB impaired colony formation and sphere formation in CRC cells, accompanied by an inhibition of colon tumor growth. Further results suggested that ATB regulated the transcriptional activity of the β‑catenin pathway by inhibiting β‑catenin expression. In addition, the results confirmed the role of β‑catenin in ATB‑mediated regulation of stemness in CRC. Collectively, the results indicated that ATB is a promising therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Tao , Wang C, Chen W, Hua F and Qian H: lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway. Exp Ther Med 19: 3097-3103, 2020.
APA
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., & Qian, H. (2020). lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway. Experimental and Therapeutic Medicine, 19, 3097-3103. https://doi.org/10.3892/etm.2020.8558
MLA
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., Qian, H."lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway". Experimental and Therapeutic Medicine 19.4 (2020): 3097-3103.
Chicago
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., Qian, H."lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3097-3103. https://doi.org/10.3892/etm.2020.8558
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Tao , Wang C, Chen W, Hua F and Qian H: lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway. Exp Ther Med 19: 3097-3103, 2020.
APA
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., & Qian, H. (2020). lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway. Experimental and Therapeutic Medicine, 19, 3097-3103. https://doi.org/10.3892/etm.2020.8558
MLA
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., Qian, H."lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway". Experimental and Therapeutic Medicine 19.4 (2020): 3097-3103.
Chicago
Yang, X., Tao, ., Wang, C., Chen, W., Hua, F., Qian, H."lncRNA‑ATB promotes stemness maintenance in colorectal cancer by regulating transcriptional activity of the β‑catenin pathway". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3097-3103. https://doi.org/10.3892/etm.2020.8558
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