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DNA‑methylated gene markers for colorectal cancer in TCGA database

  • Authors:
    • Hui Zhang
    • Xun Sun
    • Ya Lu
    • Jianzhong Wu
    • Jifeng Feng
  • View Affiliations / Copyright

    Affiliations: Research Center for Clinical Oncology, The Affiliated Cancer Hospital of Nanjing Medical University, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing, Jiangsu 210000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3042-3050
    |
    Published online on: February 27, 2020
       https://doi.org/10.3892/etm.2020.8565
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Abstract

Colorectal cancer (CRC) is characterized by the accumulation of genetic and epigenetic alterations in neoplastic processes. DNA methylation, as an important epigenetic process, contributes to the development of CRC. In the present study, the epigenetic landscape of genes in CRC was characterized by analyzing the dataset from The Cancer Genome Atlas database and 177 DNA‑methylated genes were screened based on the criterion of the Pearson correlation (R) between expression and methylation levels being >0.4. Pathway enrichment analysis revealed prominent pathways, including transcription and metabolism, further implying their significant role in tumorigenesis. Among the methylated genes, only zinc finger protein (ZNF)726 with aberrant expression was determined to affect overall survival (OS) as well as disease‑free survival of patients with CRC. In addition, ZNF726 was identified as an independent prognostic risk factor for OS in patients with CRC. The methylation‑based regulation of ZNF726 expression in CRC cells was further assessed using the Cancer Cell Line Encyclopedia database. Finally, the CpG island methylation of the ZNF726 promoter was evaluated to further elucidate its role in the development of CRC. In conclusion, the epigenetic landscape of genes in terms of promoter methylation in CRC was characterized, revealing that aberrant expression of ZNF726 may be an independent prognostic risk factor for OS in patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Sun X, Lu Y, Wu J and Feng J: DNA‑methylated gene markers for colorectal cancer in TCGA database. Exp Ther Med 19: 3042-3050, 2020.
APA
Zhang, H., Sun, X., Lu, Y., Wu, J., & Feng, J. (2020). DNA‑methylated gene markers for colorectal cancer in TCGA database. Experimental and Therapeutic Medicine, 19, 3042-3050. https://doi.org/10.3892/etm.2020.8565
MLA
Zhang, H., Sun, X., Lu, Y., Wu, J., Feng, J."DNA‑methylated gene markers for colorectal cancer in TCGA database". Experimental and Therapeutic Medicine 19.4 (2020): 3042-3050.
Chicago
Zhang, H., Sun, X., Lu, Y., Wu, J., Feng, J."DNA‑methylated gene markers for colorectal cancer in TCGA database". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3042-3050. https://doi.org/10.3892/etm.2020.8565
Copy and paste a formatted citation
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Spandidos Publications style
Zhang H, Sun X, Lu Y, Wu J and Feng J: DNA‑methylated gene markers for colorectal cancer in TCGA database. Exp Ther Med 19: 3042-3050, 2020.
APA
Zhang, H., Sun, X., Lu, Y., Wu, J., & Feng, J. (2020). DNA‑methylated gene markers for colorectal cancer in TCGA database. Experimental and Therapeutic Medicine, 19, 3042-3050. https://doi.org/10.3892/etm.2020.8565
MLA
Zhang, H., Sun, X., Lu, Y., Wu, J., Feng, J."DNA‑methylated gene markers for colorectal cancer in TCGA database". Experimental and Therapeutic Medicine 19.4 (2020): 3042-3050.
Chicago
Zhang, H., Sun, X., Lu, Y., Wu, J., Feng, J."DNA‑methylated gene markers for colorectal cancer in TCGA database". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3042-3050. https://doi.org/10.3892/etm.2020.8565
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