LINC00210 plays oncogenic roles in non‑small cell lung cancer by sponging microRNA‑328‑5p

Liu,Zhengjia; Xu,Lei; Zhang,Kejian; Guo,Bo; Cui,Zhi; Gao,Nan

doi:10.3892/etm.2020.8593

LINC00210 plays oncogenic roles in non‑small cell lung cancer by sponging microRNA‑328‑5p

Authors:
- Zhengjia Liu
- Lei Xu
- Kejian Zhang
- Bo Guo
- Zhi Cui
- Nan Gao
View Affiliations

Affiliations: Department of Thoracic Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Thoracic Surgery, Jilin Cancer Hospital, Changchun, Jilin 130021, P.R. China
Published online on: March 11, 2020 https://doi.org/10.3892/etm.2020.8593
Pages: 3325-3331
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Long noncoding RNA (lncRNA) has an important role in regulating non‑small cell lung cancer (NSCLC) progression. The present study aimed to investigate the effect of LINC00210 in NSCLC progression in order to provide a novel treatment target for patients with NSCLC. A total of 39 NSCLC patients were obtained and divided into LINC00210 high expression and low expression groups. Subsequently, the 5‑year survival rate from this patient cohort was analyzed. The SK‑MES‑1 and A549 NSCLC and the human 16‑HBE bronchial epithelial cell lines were utilized to investigate expression level of LIN00210. A549 cells were used to investigate cell proliferation, migration and invasive abilities using Cell Counting kit 8, Transwell and Matrigel assays, respectively. In addition, the luciferase reporter gene assay was performed to investigate the potential target of LINC00210. Reverse transcription‑quantitative PCR was used to determine LINC00210 and microRNA (miR)‑328‑5p expression levels in NSCLC tissues and tumor cell lines (SK‑MES‑1 and A549). The results demonstrated that LINC00210 was upregulated in NSCLC tissues and cell lines compared with that in normal tissues and 16‑HBE cells, and that LINC00210 expression was associated with a poor prognosis in patients with NSCLC (P<0.05). Furthermore, A549 cell transfection with small interfering (si)LINC00210#1 and siLINC00210#2 induced a significant decrease in cell proliferation, and migratory and invasive abilities compared with that in the control groups (P<0.05). In addition, miR‑328‑5p overexpression was stimulated by knockdown of LINC00210. Furthermore, A549 cells transfected with siLINC00210#1 and miR‑328‑5p inhibitor exhibited a significant increase in cell proliferation, and migratory and invasive ability compared with that in A549 cells transfected with siLINC00210#1. These findings suggest that LINC00210 may serve as an oncogenic role in NSCLC by sponging miR‑328‑5p.

View Figures

View References

1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017.PubMed/NCBI View Article : Google Scholar
2	Travis WD: The 2015 WHO classification of lung tumors. Der Pathologe. 35 (Suppl 2)(S188)2014.
3	Meseure D, Drak Alsibai K, Nicolas A, Bieche I and Morillon A: Long noncoding RNAs as new architects in cancer epigenetics, prognostic biomarkers, and potential therapeutic targets. Biomed Res Int. 2015(320214)2015.PubMed/NCBI View Article : Google Scholar
4	Yao Y, Li J and Wang L: Large intervening non-coding RNA HOTAIR is an indicator of poor prognosis and a therapeutic target in human cancers. Int J Mol Sci. 15:18985–18999. 2014.PubMed/NCBI View Article : Google Scholar
5	Liu Z, Sun M, Lu K, Liu J, Zhang M, Wu W, Wei D, Wang Z and Wang R: The long noncoding RNA HOTAIR contributes to cisplatin resistance of human lung adenocarcinoma cells via downregualtion of p21 WAF1/CIP1 expression. PLoS One. 8(e77293)2013.PubMed/NCBI View Article : Google Scholar
6	Cheng N, Cai W, Ren S, Li X, Wang Q, Pan H, Zhao M, Li J, Zhang Y, Zhao C, et al: Long non-coding RNAUCA1induces non-T790M acquired resistance to EGFR-TKIs by activating the AKT/mTOR pathway in EGFR-mutant non-small cell lung cancer. Oncotarget. 6:23582–23593. 2015..v. PubMed/NCBI View Article : Google Scholar
7	Guo F, Jiao F, Song Z, Li S, Liu B, Yang H, Zhou Q and Li Z: Regulation of MALAT1 expression by TDP43 controls the migration and invasion of non-small cell lung cancer cells in vitro. Biochem Biophys Res Commun. 465:293–298. 2015.PubMed/NCBI View Article : Google Scholar
8	Wu D, Li Y, Zhang H and Hu X: Knockdown of Lncrna PVT1 enhances radiosensitivity in non-small cell lung cancer by sponging Mir-195. Cell Physiol Biochem. 42:2453–2466. 2017.PubMed/NCBI View Article : Google Scholar
9	Zhang S, Li P, Zhao L and Xu L: LINC00210 as a miR-328-5p sponge promotes nasopharyngeal carcinoma tumorigenesis by activating NOTCH3 pathway. Biosci Rep. 38(BSR20181168)2018.PubMed/NCBI View Article : Google Scholar
10	Amin MB, Greene FL, Edge SB, Compton CC, Gershenwald JE, Brookland RK, Meyer L, Gress DM, Byrd DR and Winchester DP: The eighth edition AJCC Cancer Staging Manual: Continuing to build a bridge from a population-based to a more ‘personalized’ approach to cancer staging. CA Cancer J Clin. 67:93–99. 2017.PubMed/NCBI View Article : Google Scholar
11	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
12	Geng C, Ziyun W, Dongqing W, Chengxiang Q, Mingxi L, Xing C, Qipeng Z, Guiying Y and Qinghua C: LncRNADisease: A database for long-non-coding RNA-associated diseases. Nucleic Acids Res. 41 (Database Issue):D983–D986. 2013.PubMed/NCBI View Article : Google Scholar
13	Li N, Wang Y, Liu X, Luo P, Jing W, Zhu M and Tu J: Identification of circulating long noncoding RNA HOTAIR as a novel biomarker for diagnosis and monitoring of non-small cell lung cancer. Technol Cancer Res Treat. 16:1060–1066. 2017.PubMed/NCBI View Article : Google Scholar
14	Li S, Mei Z, Hu HB and Zhang X: The lncRNA MALAT1 contributes to non-small cell lung cancer development via modulating miR-124/STAT3 axis. J Cell Physiol. 233:6679–6688. 2017.PubMed/NCBI View Article : Google Scholar
15	Zhao Z, Wang J, Wang S, Chang H, Zhang T and Qu J: LncRNA CCAT2 promotes tumorigenesis by over-expressed Pokemon in non-small cell lung cancer. Biomed Pharmacother. 87:692–697. 2017.PubMed/NCBI View Article : Google Scholar
16	Huang Z, Lei W, Hu HB, Zhang H and Zhu Y: H19 promotes non-small-cell lung cancer (NSCLC) development through STAT3 signaling via sponging miR-17. J Cell Physiol. 233:6768–6776. 2018.PubMed/NCBI View Article : Google Scholar
17	Deng J, Liang Y, Liu C, He S and Wang S: The up-regulation of long non-coding RNA AFAP1-AS1 is associated with the poor prognosis of Patients with NSCLC. Biomed Pharmacother. 75:8–11. 2015.PubMed/NCBI View Article : Google Scholar
18	Sun M, Liu XH, Lu KH, Nie FQ, Xia R, Kong R, Yang JS, Xu TP, Liu YW, Zou YF, et al: EZH2-mediated epigenetic suppression of long noncoding RNA SPRY4-IT1 promotes NSCLC cell proliferation and metastasis by affecting the epithelial-mesenchymal transition. Cell Death Dis. 5(e1298)2014.PubMed/NCBI View Article : Google Scholar
19	Lu KH, Li W, Liu XH, Sun M, Zhang ML, Wu WQ, Xie WP and Hou YY: Long non-coding RNA MEG3 inhibits NSCLC cells proliferation and induces apoptosis by affecting p53 expression. BMC Cancer. 13(461)2013.PubMed/NCBI View Article : Google Scholar
20	Han L, Kong R, Yin DD, Zhang EB, Xu TP and De W Shu YQ: Low expression of long noncoding RNA GAS6-AS1 predicts a poor prognosis in patients with NSCLC. Med Oncol. 30(694)2013.PubMed/NCBI View Article : Google Scholar
21	Mei Y, Si J, Wang Y, Huang Z, Zhu H, Feng S, Wu X and Wu L: Long noncoding RNA GAS5 suppresses tumorigenesis by inhibiting miR-23a 5 expression in non-small cell lung cancer. Oncol Res. 25:1027–1037. 2017.PubMed/NCBI View Article : Google Scholar
22	Han L, Zhang E, Yin D, Kong R, Xu T, Chen W, Xia R, Shu Y and De W: Low expression of long noncoding RNA PANDAR predicts a poor prognosis of non-small cell lung cancer and affects cell apoptosis by regulating Bcl-2. Cell Death Dis. 6(e1665)2015.PubMed/NCBI View Article : Google Scholar
23	Fu X, Zhu X, Qin F, Zhang Y, Lin J, Ding Y, Yang Z, Shang Y, Wang L, Zhang Q and Gao Q: Linc00210 drives Wnt/β-catenin signaling activation and liver tumor progression through CTNNBIP1-dependent manner. Mol Cancer. 17(73)2018.PubMed/NCBI View Article : Google Scholar
24	Li J, Li Q, Huang H, Li Y, Li L, Hou W and You Z: Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. Int J Oncol. 50:1087–1096. 2017.PubMed/NCBI View Article : Google Scholar
25	Santasusagna S, Moreno I, Navarro A, Muñoz C, Martinez F, Hernández R, Castellano JJ and Monzo M: miR-328 mediates a metabolic shift in colon cancer cells by targeting SLC2A1/GLUT1. Clin Transl Oncol. 20:1161–1167. 2018.PubMed/NCBI View Article : Google Scholar
26	Lin CH, Chiang MC and Chen YJ: MicroRNA-328 inhibits migration and epithelial-mesenchymal transition by targeting CD44 in nasopharyngeal carcinoma cells. Onco Targets Ther. 11:2375–2385. 2018.PubMed/NCBI View Article : Google Scholar
27	Liu L, Chen RA, Zhang Y, Fan W, Xiao F and Yan X: Low expression of circulating microRNA-328 is associated with poor prognosis in patients with acute myeloid leukemia. Diagn Pathol. 10(109)2015.PubMed/NCBI View Article : Google Scholar
28	Yuan J, Zheng Z, Zheng Y, Lu X, Xu L and Lin L: microRNA-328 is a favorable prognostic marker in human glioma via suppressing invasive and proliferative phenotypes of malignant cells. Int J Neurosci. 126:145–153. 2016.PubMed/NCBI View Article : Google Scholar
29	Wu Z, Sun L, Wang H, Yao J, Jiang C, Xu W and Yang Z: MiR-328 expression is decreased in high-grade gliomas and is associated with worse survival in primary glioblastoma. PLoS One. 7(e47270)2012.PubMed/NCBI View Article : Google Scholar
30	Ma W, Ma CN, Zhou NN, Li XD and Zhang YJ: Up-regulation of miR-328-3p sensitizes non-small cell lung cancer to radiotherapy. Sci Rep. 6(31651)2016.PubMed/NCBI View Article : Google Scholar