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Article

Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1

  • Authors:
    • Zhigang Zhao
    • Zhongzhi Tang
    • Wenkai Zhang
    • Jie Liu
    • Bo Li
    • Shifang Ding
  • View Affiliations / Copyright

    Affiliations: Emergency Department, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430000, P.R. China, Emergency Department, General Hospital of Central Theater Command, Wuhan, Hubei 430070, P.R. China, Cardiovascular Department, General Hospital of Central Theater Command, Wuhan, Hubei 430070, P.R. China
  • Pages: 3362-3368
    |
    Published online on: March 17, 2020
       https://doi.org/10.3892/etm.2020.8605
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Abstract

The current study investigated the protective effects of inactivated pseudomonas aeruginosa (IPA) on myocardial ischemia reperfusion injury (MIR/I) and the mechanisms governing this interaction. Left anterior descending coronary artery ligation was performed on rats for 30 min and reperfusion was performed for a subsequent 2 h. Rat hearts were obtained and the myocardial infarction area was determined using nitroblue tetrazolium. Myocardial cell apoptosis was determined using flow cytometry. Malondialdehyde (MDA) content, lactate dehydrogenase (LDH) activity, superoxide dismutase (SOD) activity and catalase (CAT) activities were assayed using the corresponding kits. Additionally, nuclear factor erythroid 2‑related factor 2 (Nrf2) and heme oxygenase 1 (HO‑1) were assayed using western blot and immunofluorescence analysis. When compared with the model group, the results of IPA treatment revealed improved heart function, reduced myocardial infarction area and reduced endothelial cell apoptosis, which led to decreased LDH and MDA levels, and increased SOD and CAT levels in serum, and decreased LDH and MDA levels and increased SOD and CAT in myocardial tissues. Moreover, increased Nrf2 and HO‑1 expression levels in the myocardial tissues were also observed at all concentrations of IPA. It was concluded that IPA pretreatment ameliorated MIR/I and reduced endothelial apoptosis and oxidative stress via the Nrf2/HO‑1 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Z, Tang Z, Zhang W, Liu J, Li B and Ding S: Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1. Exp Ther Med 19: 3362-3368, 2020.
APA
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., & Ding, S. (2020). Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1. Experimental and Therapeutic Medicine, 19, 3362-3368. https://doi.org/10.3892/etm.2020.8605
MLA
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., Ding, S."Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1". Experimental and Therapeutic Medicine 19.5 (2020): 3362-3368.
Chicago
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., Ding, S."Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1". Experimental and Therapeutic Medicine 19, no. 5 (2020): 3362-3368. https://doi.org/10.3892/etm.2020.8605
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Z, Tang Z, Zhang W, Liu J, Li B and Ding S: Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1. Exp Ther Med 19: 3362-3368, 2020.
APA
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., & Ding, S. (2020). Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1. Experimental and Therapeutic Medicine, 19, 3362-3368. https://doi.org/10.3892/etm.2020.8605
MLA
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., Ding, S."Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1". Experimental and Therapeutic Medicine 19.5 (2020): 3362-3368.
Chicago
Zhao, Z., Tang, Z., Zhang, W., Liu, J., Li, B., Ding, S."Inactivated pseudomonas aeruginosa protects against myocardial ischemia reperfusion injury via Nrf2 and HO‑1". Experimental and Therapeutic Medicine 19, no. 5 (2020): 3362-3368. https://doi.org/10.3892/etm.2020.8605
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