Protective effects of postconditioning in transvaginally created pneumoperitoneum

Shanava,Koba; Javor,Szaniszlo; Kerkadze,Vakhtang; Abiatari,Ivane; Weber,György

doi:10.3892/etm.2020.8616

Protective effects of postconditioning in transvaginally created pneumoperitoneum

Authors:
- Koba Shanava
- Szaniszlo Javor
- Vakhtang Kerkadze
- Ivane Abiatari
- György Weber
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Affiliations: Institute of Medical Research, Ilia State University, Tbilisi 0162, Georgia, Department of Surgical Research and Techniques, Medical Faculty, University of Pecs, H‑7624 Pécs, Hungary
Published online on: March 19, 2020 https://doi.org/10.3892/etm.2020.8616
Pages: 3861-3866

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Abstract

There are reports of ischemic complications in clinical practice after laparoscopy using pneumoperitoneum. Conditioning has a beneficial effect for various ischemic diseases. This experimental study was designed to evaluate the effects of postconditioning in transvaginally created pneumoperitoneum. Sixty adult female rats, weighing 300±50 g were divided into four equal groups. Pneumoperitoneum was created by CO2 insufflation under a pressure of 10 mmHg. Rats in the first group (sham) were subjected to only sham‑operation or gas insufflation. The second group (TV/PP) was subjected to pneumoperitoneum for 60 min followed by 30 min of desufflation. The third group (post‑5) was subjected to pneumoperitoneum for 60 min followed by 5 min of desufflation, 5 min of insufflation and again followed by 30 min of desufflation. The fourth group (post‑2.5) was subjected to pneumoperitoneum for 60 min followed by 2.5 min of desufflation and 2.5 min of insufflation‑repeated in two cycles‑ and then followed by 30 min of desufflation. The rats were sacrificed, and blood was collected after 30 min, 2 and 6 h from the last desufflation. Levels of oxidative stress markers, malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), sulfhydryl groups (SH) and inflammatory cytokine TNF‑α, were analyzed. Levels of MDA in the post‑5 group were significantly reduced compared to the TV/PP and post‑2.5 groups. The level of GSH in TV/PP animals was markedly reduced compared to the Sham, Post‑5 and Post‑2.5 groups. In addition, levels of SH were increased in the Post‑5 group in comparison to the Sham, TV/PP and Post‑2.5 groups. No difference in the activity of SOD between the groups was found, and the concentration of TNF‑α in TV/PP animals was significantly higher than that in the Sham and postconditioning groups. Overall, the results of the present study indicate that postconditioning can reduce pneumoperitoneum‑induced oxidative injury.

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