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Matrix metalloproteinase‑13, NF‑κB p65 and interleukin‑1β are associated with the severity of knee osteoarthritis

  • Authors:
    • Qing‑Hua Zhao
    • Lu‑Pan Lin
    • Yu‑Xiang Guo
    • Rui Zou
    • Zhen Wang
    • Zhong‑Ping Shi
    • Fu‑Qing Lin

  • View Affiliations

  • Published online on: March 20, 2020     https://doi.org/10.3892/etm.2020.8618
  • Pages: 3620-3626

  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Knee osteoarthritis (KOA) is a prevalent disease, especially in the elderly. The present study examined the expression of matrix metalloproteinase‑13 (MMP‑13), NF‑κBp65 and interleukin (IL)‑lβ in the synovial tissues of KOA patients and the role of MMP‑13 and the NF‑κBp65 signalling pathway in KOA pathogenesis. A total of 100 KOA patients were enrolled in our hospital from December 2015 to December 2017 and were classified into either a mild KOA group (Outerbridge grade 1 and 2) or a severe KOA group (Outerbridge grade 3 and 4). Non‑OA patients were included as controls. Synovial tissues from patients in both groups were collected for detection of the mRNA and protein expression of MMP‑13, NF‑κBp65 and IL‑lβ. Synovial tissue slices were subjected to haematoxylin and eosin staining and immunohistochemistry (SP method). Cartilage tissues were observed under a light microscope after Safranin O‑fast green staining. Reverse transcription‑quantitative PCR and western blot analyses demonstrated that the expression of MMP‑13, NF‑κBp65 and IL‑lβ in the mild and severe groups were substantially upregulated compared with the control group (all P<0.05). A positive correlation between MMP‑13 and NF‑κBp65 expression in the KOA synovial tissues was identified (P<0.05). Immunohistochemistry revealed that the expression of MMP‑13 and NF‑κBp65 was related to the severity of KOA (MMP‑13: severe, 92.54%; moderate, 76.52%; control: 32.14%; and NF‑κBp65: severe, 85.56%; moderate, 48.12%; control: 28.32%). This evidence indicated that the severity of KOA was related to MMP‑13 and NF‑κBp65 expression. The NF‑κB signalling pathway may be activated during OA progression, which could upregulate the expression of MMP‑13 and IL‑1β and accelerate the deterioration of articular cartilage.
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June-2020
Volume 19 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao QH, Lin LP, Guo YX, Zou R, Wang Z, Shi ZP and Lin FQ: Matrix metalloproteinase‑13, NF‑κB p65 and interleukin‑1β are associated with the severity of knee osteoarthritis. Exp Ther Med 19: 3620-3626, 2020
APA
Zhao, Q., Lin, L., Guo, Y., Zou, R., Wang, Z., Shi, Z., & Lin, F. (2020). Matrix metalloproteinase‑13, NF‑κB p65 and interleukin‑1β are associated with the severity of knee osteoarthritis. Experimental and Therapeutic Medicine, 19, 3620-3626. https://doi.org/10.3892/etm.2020.8618
MLA
Zhao, Q., Lin, L., Guo, Y., Zou, R., Wang, Z., Shi, Z., Lin, F."Matrix metalloproteinase‑13, NF‑κB p65 and interleukin‑1β are associated with the severity of knee osteoarthritis". Experimental and Therapeutic Medicine 19.6 (2020): 3620-3626.
Chicago
Zhao, Q., Lin, L., Guo, Y., Zou, R., Wang, Z., Shi, Z., Lin, F."Matrix metalloproteinase‑13, NF‑κB p65 and interleukin‑1β are associated with the severity of knee osteoarthritis". Experimental and Therapeutic Medicine 19, no. 6 (2020): 3620-3626. https://doi.org/10.3892/etm.2020.8618