microRNA‑146b promotes neuroblastoma cell growth through targeting NUMB
Retraction in: /10.3892/etm.2024.12424
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- Published online on: March 23, 2020 https://doi.org/10.3892/etm.2020.8623
- Pages: 3531-3536
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Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Accumulating evidence has demonstrated that the abnormal expression of microRNA (miRNA/miR) serves a crucial role in the development of numerous types of human cancer, including neuroblastoma (NB). The present study aimed to investigate the expression levels and biological roles of miR‑146b in NB. miR‑146b expression levels in NB cell lines and human umbilical vein endothelial cells (HUVECs) were analyzed using reverse transcription‑quantitative PCR, and the regulatory effects of miR‑146b on NB cell proliferation, invasion and apoptosis in vitro were investigated using CCK‑8 assay, transwell invasion assay and flow cytometry. In addition, bioinformatics analysis, western blotting and dual‑luciferase reporter assays were used to determine whether NUMB was a target gene of miR‑146b. miR‑146b expression levels were increased in NB cell lines compared with HUVECs. The knockdown of miR‑146b using a miR‑146b inhibitor significantly inhibited NB cell proliferation and invasion, but promoted cell apoptosis in vitro. Furthermore, it was revealed that miR‑146b promoted NB cell proliferation through targeting NUMB. In conclusion, miR‑146b was suggested to serve as an oncogene, at least in part, through directly targeting NUMB, which indicated that miR‑146b may be a potential therapeutic target for NB treatment.
