miR-135b levels in the peripheral blood serve as a marker associated with acute ischemic stroke
- Sha Yang
- Xinyu Zhan
- Min He
- Jingjing Wang
- Xuemei Qiu
Affiliations: Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China, Vasculocardiology Department, The 903rd Hospital of PLA, Hangzhou, Zhejiang 310013, P.R. China, Department of Clinical Laboratory Medicine, The 80th Army Hospital of PLA, Weifang, Shandong 261021, P.R. China
- Published online on: March 30, 2020 https://doi.org/10.3892/etm.2020.8628
Copyright: © Yang
et al. This is an open access article distributed under the
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The protective role of microRNA (miR)-135b in cerebral neurons has been previously identified. However, to the best of our knowledge, the involvement of miR-135b in acute ischemic stroke has yet to be elucidated. The present study aimed to investigate the expression profile of miR-135b in peripheral blood obtained from patients with acute ischemic stroke. A total of 76 patients with acute ischemic stroke were selected as the case group, which included 33 cases of aorta atheromatous plague, 19 cases of cardioembolism, 16 cases of small arterial occlusion and 8 cases with unknown causes. In addition, 60 healthy subjects were selected as the control group. Reverse transcription-quantitative PCR was used to measure the expression of miR-135b in the peripheral blood of the patients. The National Institutes of Health Stroke Scale (NIHSS) score was used to evaluate the severity of acute ischemic stroke. The relationship between miR-135b levels and acute stroke was subsequently analyzed. The expression of miR-135b in the peripheral blood of the case group was found to be significantly higher compared with that in the control group. By contrast, the expression levels of miR-135b in the case group did not differ significantly between the different etiology types of acute ischemic stroke. In addition, a significant positive correlation was observed between levels of miR-135b expression and NIHSS scores. Further analysis demonstrated that hypertension, hyperglycemia, platelet count, international normalized ratio and miR-135b were risk factors for acute ischemic stroke. Based on bioinformatics analysis, a conserved binding site for miR-135b was identified in the 3'-untranslated region of the transient receptor potential cation channel subfamily C member 6 (TRPC6). Dual luciferase reporter and western blot analysis showed that TRPC6 was a target gene of miR-135b. In conclusion, data from the present study suggest that elevated expression of miR-135b in the peripheral blood of patients with acute ischemic stroke is closely associated with disease severity.