Liraglutide ameliorates COCl2-induced oxidative stress and apoptosis in H9C2 cells via regulating cell autophagy

Pang,Zhanqi; Wang,Tao; Li,Yawen; Wang,Lin; Yang,Jian; Dong,He; Li,Shijun

doi:10.3892/etm.2020.8630

Liraglutide ameliorates COCl2-induced oxidative stress and apoptosis in H9C2 cells via regulating cell autophagy

Authors:
- Zhanqi Pang
- Tao Wang
- Yawen Li
- Lin Wang
- Jian Yang
- He Dong
- Shijun Li
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Affiliations: Department of Cardiology, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 116033, P.R. China
Published online on: March 31, 2020 https://doi.org/10.3892/etm.2020.8630
Pages: 3716-3722
Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Protective effects of liraglutide on H9C2 cells cultured using CoCl2 and its mechanism of action were investigated. Hypoxia model was established using CoCl2‑treated H9C2 cells. With liraglutide as the treatment factor, apoptosis, changes in nitric oxide (NO) and reactive oxygen species (ROS) activity, mitochondrial membrane potential and change in cell autophagy level were detected via Hoechst staining, enzyme‑linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and western blotting (WB), respectively. Liraglutide ameliorated the CoCl2‑induced decrease in H9C2 cell viability, the increases in cytotoxicity and percentage of apoptotic cells as well as oxidative stress in cells. Moreover, it stimulated the elevation of cell autophagy level. However, the protective effects of liraglutide on H9C2 cells were attenuated remarkably after adding the cell autophagy inhibitor. Liraglutide can ameliorate the CoCl2‑induced oxidative stress and apoptosis in H9C2 cells via regulating cell autophagy.

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