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Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis

  • Authors:
    • Kun Chen
    • Yanchun Ma
    • Tianling Ding
    • Xinju Zhang
    • Bobin Chen
    • Ming Guan
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Huashan Hospital North, Fudan University, Shanghai 201907, P.R. China, Department of Hematology, Huashan Hospital North, Fudan University, Shanghai 201907, P.R. China, Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 301-308
    |
    Published online on: April 29, 2020
       https://doi.org/10.3892/etm.2020.8695
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Abstract

Primary central nervous system lymphoma (PCNSL) is a rare type of primary extranodal lymphoma (PEL). MYD88L265P mutation has been observed in up to 75% of PCNSL cases, however, the validity and sensitivity of digital PCR in detecting this mutation remains to be elucidated. A total of 44 PCNSL patients, 15 diffuse large B‑cell lymphoma not otherwise specified (DLBCL‑NOS) patients and 13 other PEL patients were enrolled in the present study. The abilities of reverse transcription quantitative PCR (RT‑qPCR) and droplet digital PCR (ddPCR) to detect the MYD88L265P mutation in cerebral spinal fluid (CSF) samples were compared. The results suggested that ddPCR showed superior mutation detection sensitivity when compared with RT‑qPCR (58 vs. 15%; P<0.05). The MYD88L265P mutation was significantly associated with increased MYD88 protein overexpression in PCNSL brain tissue samples (P<0.05). Analysis of MYD88L265P mutation status in CSF and vitreous fluid samples using ddPCR may be a promising technique for minimally invasive confirmation of PCNSL diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen K, Ma Y, Ding T, Zhang X, Chen B and Guan M: Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis. Exp Ther Med 20: 301-308, 2020.
APA
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., & Guan, M. (2020). Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis. Experimental and Therapeutic Medicine, 20, 301-308. https://doi.org/10.3892/etm.2020.8695
MLA
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., Guan, M."Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis". Experimental and Therapeutic Medicine 20.1 (2020): 301-308.
Chicago
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., Guan, M."Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis". Experimental and Therapeutic Medicine 20, no. 1 (2020): 301-308. https://doi.org/10.3892/etm.2020.8695
Copy and paste a formatted citation
x
Spandidos Publications style
Chen K, Ma Y, Ding T, Zhang X, Chen B and Guan M: Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis. Exp Ther Med 20: 301-308, 2020.
APA
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., & Guan, M. (2020). Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis. Experimental and Therapeutic Medicine, 20, 301-308. https://doi.org/10.3892/etm.2020.8695
MLA
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., Guan, M."Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis". Experimental and Therapeutic Medicine 20.1 (2020): 301-308.
Chicago
Chen, K., Ma, Y., Ding, T., Zhang, X., Chen, B., Guan, M."Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis". Experimental and Therapeutic Medicine 20, no. 1 (2020): 301-308. https://doi.org/10.3892/etm.2020.8695
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