Promoter hypermethylation influences the suppressive role of long non‑coding RNA MEG3 in the development of multiple myeloma

  • Authors:
    • Wenjun Yu
    • Qinglin Shi
    • Chao Wu
    • Xuxing Shen
    • Lijuan Chen
    • Jiaren Xu
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  • Published online on: May 6, 2020     https://doi.org/10.3892/etm.2020.8723
  • Pages: 637-645
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Abstract

Methylation is a fundamental regulator of gene transcription. Long non-coding RNA maternally expressed 3 (MEG3) inhibits cell proliferation in various types of cancer. However, the molecular mechanisms of MEG3 methylation in the regulation of multiple myeloma (MM) are unknown. In the present study, MEG3 upregulation was negatively associated with the International Staging System (ISS) status of the bone marrow samples of 39 patients with MM. MEG3 overexpression in an MM cell line resulted in elevated p53 expression. Furthermore, the results of methylation‑specific PCR revealed that the abnormal methylation status of the MEG3 promoter region was present in eight of the 39 bone marrow samples collected. Treatment of the MM cell line with the DNA methylation inhibitor 5‑Aza‑2'‑deoxycytidine (5‑Aza‑CdR) resulted in tumor cell proliferation inhibition, apoptosis induction and G0/G1 cell cycle arrest. Furthermore, 5‑Aza‑CdR decreased aberrant hypermethylation of the MEG3 promoter and increased the expression of MEG3. However, 5‑Aza‑CdR exerted no effect on p53 expression. To the best of our knowledge, the present study is the first to report that the demethylation reagent 5‑Aza‑CdR may serve as a therapeutic agent in MM by upregulating MEG3 expression. However, the mechanism of action was independent of p53 expression.
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July-2020
Volume 20 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yu W, Shi Q, Wu C, Shen X, Chen L and Xu J: Promoter hypermethylation influences the suppressive role of long non‑coding RNA MEG3 in the development of multiple myeloma. Exp Ther Med 20: 637-645, 2020
APA
Yu, W., Shi, Q., Wu, C., Shen, X., Chen, L., & Xu, J. (2020). Promoter hypermethylation influences the suppressive role of long non‑coding RNA MEG3 in the development of multiple myeloma. Experimental and Therapeutic Medicine, 20, 637-645. https://doi.org/10.3892/etm.2020.8723
MLA
Yu, W., Shi, Q., Wu, C., Shen, X., Chen, L., Xu, J."Promoter hypermethylation influences the suppressive role of long non‑coding RNA MEG3 in the development of multiple myeloma". Experimental and Therapeutic Medicine 20.1 (2020): 637-645.
Chicago
Yu, W., Shi, Q., Wu, C., Shen, X., Chen, L., Xu, J."Promoter hypermethylation influences the suppressive role of long non‑coding RNA MEG3 in the development of multiple myeloma". Experimental and Therapeutic Medicine 20, no. 1 (2020): 637-645. https://doi.org/10.3892/etm.2020.8723