Polymyxin B resistance rates in carbapenem‑resistant Pseudomonas aeruginosa isolates and a comparison between Etest® and broth microdilution methods of antimicrobial susceptibility testing

  • Authors:
    • Xu Chen
    • Jie Xu
    • Qiongfang Zhu
    • Yalu Ren
    • Lina Zhao
  • View Affiliations

  • Published online on: May 20, 2020     https://doi.org/10.3892/etm.2020.8777
  • Pages: 762-769
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Abstract

Polymyxin B has been considered to be the last line of defense for life-threatening infections caused by multiple drug resistant gram‑negative pathogens, including carbapenem‑resistant Pseudomonas aeruginosa (CRPA). The present study analyzed CRPA resistance to polymyxin B in the Suzhou district of China. Additionally, polymyxin B resistance rates were compared in different parts of the world to determine global trends. The present study also assessed the reliability and effectiveness of the Etest® in a clinical setting, as laboratories lack a reliable and efficient susceptibility test for polymyxin B. The susceptibility rate of polymyxin B reached 96.0%, which is in accordance with results obtained from the United States of America, Europe, Africa and the majority of Asian countries. However, the rate of polymyxin B non‑susceptibility (resistant or intermediate) in Singapore is 0.53 (95% confidence interval, 0.12‑0.93). In addition, the susceptibility rate of polymyxin B determined via Etest® was not significantly increased compared with that determined via broth microdilution (98.0 vs. 96.0%; P=0.558). Essential and categorical agreement rates reached 98.0%. In conclusion, the polymyxin B resistance rate of CRPA isolates is relatively low in the majority of countries, with the exception of Singapore. Furthermore, Etest® may be a reliable clinical method for the measurement of polymyxin B resistance in CRPA isolates.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen X, Xu J, Zhu Q, Ren Y and Zhao L: Polymyxin B resistance rates in carbapenem‑resistant Pseudomonas aeruginosa isolates and a comparison between Etest® and broth microdilution methods of antimicrobial susceptibility testing. Exp Ther Med 20: 762-769, 2020
APA
Chen, X., Xu, J., Zhu, Q., Ren, Y., & Zhao, L. (2020). Polymyxin B resistance rates in carbapenem‑resistant Pseudomonas aeruginosa isolates and a comparison between Etest® and broth microdilution methods of antimicrobial susceptibility testing. Experimental and Therapeutic Medicine, 20, 762-769. https://doi.org/10.3892/etm.2020.8777
MLA
Chen, X., Xu, J., Zhu, Q., Ren, Y., Zhao, L."Polymyxin B resistance rates in carbapenem‑resistant Pseudomonas aeruginosa isolates and a comparison between Etest® and broth microdilution methods of antimicrobial susceptibility testing". Experimental and Therapeutic Medicine 20.2 (2020): 762-769.
Chicago
Chen, X., Xu, J., Zhu, Q., Ren, Y., Zhao, L."Polymyxin B resistance rates in carbapenem‑resistant Pseudomonas aeruginosa isolates and a comparison between Etest® and broth microdilution methods of antimicrobial susceptibility testing". Experimental and Therapeutic Medicine 20, no. 2 (2020): 762-769. https://doi.org/10.3892/etm.2020.8777