Open Access

Trp53 regulates platelets in bone marrow via the PI3K pathway

  • Authors:
    • Mingming Yang
    • Qing Liu
    • Ting Niu
    • Jianbiao Kuang
    • Xiaohan Zhang
    • Lingbi Jiang
    • Siqi Li
    • Xiaodong He
    • Lijing Wang
    • Jiangchao Li
  • View Affiliations

  • Published online on: June 9, 2020     https://doi.org/10.3892/etm.2020.8850
  • Pages: 1253-1260
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The p53 gene is well known as a key tumor suppressor gene; it is vital for hematopoietic stem cell differentiation and growth. In the present study, the change of platelets (PLTs) in p53 knockout mice (p53‑/‑ mice) was investigated. The peripheral blood cell subsets and PLT parameters in p53‑/‑mice were compared with those in age‑matched p53+/+ mice. Bleeding time as well as the alteration of PLT levels, were analyzed with the PLT marker CD41 antibody using flow cytometry. The results revealed that the number of PLTs in p53‑/‑ mice was significantly lower than that in p53+/+ mice. Bleeding time was prolonged in the peripheral blood of p53‑/‑ mice compared with that of p53+/+ mice. Furthermore, the related gene expression of the PI3K signaling pathway in the bone marrow of p53‑/‑ mice was shown to be associated with plateletogenesis. PI3K inhibitor (LY294002) was also used to treat p53‑/‑ mice, and the results demonstrated that LY294002 revert the change of PLTs in these mice. In summary, PLTs were altered in p53‑/‑ mice, and the PI3K signaling pathway was involved in that process, suggesting that the p53‑dependent PI3K signaling pathway is involved in thrombocytopenia or PLT diseases. PLT number is reduced in p53 deficiency; however, this reduction could be reverted by inhibiting the PI3K pathway.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Yang, M., Liu, Q., Niu, T., Kuang, J., Zhang, X., Jiang, L. ... Li, J. (2020). Trp53 regulates platelets in bone marrow via the PI3K pathway. Experimental and Therapeutic Medicine, 20, 1253-1260. https://doi.org/10.3892/etm.2020.8850
MLA
Yang, M., Liu, Q., Niu, T., Kuang, J., Zhang, X., Jiang, L., Li, S., He, X., Wang, L., Li, J."Trp53 regulates platelets in bone marrow via the PI3K pathway". Experimental and Therapeutic Medicine 20.2 (2020): 1253-1260.
Chicago
Yang, M., Liu, Q., Niu, T., Kuang, J., Zhang, X., Jiang, L., Li, S., He, X., Wang, L., Li, J."Trp53 regulates platelets in bone marrow via the PI3K pathway". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1253-1260. https://doi.org/10.3892/etm.2020.8850